أعرض في EDS

VaxiPatch™, a novel vaccination system comprised of subunit antigens, adjuvants and microneedle skin delivery: An application to influenza B/Colorado/06/2017

التفاصيل البيبلوغرافية
العنوان: VaxiPatch™, a novel vaccination system comprised of subunit antigens, adjuvants and microneedle skin delivery: An application to influenza B/Colorado/06/2017
المؤلفون: George Talbott, Thomas J. Ellison, Daniel R. Henderson
المصدر: Vaccine
سنة النشر: 2020
مصطلحات موضوعية: medicine.medical_treatment, 030231 tropical medicine, Antibodies, Viral, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Antigen, Adjuvants, Immunologic, Influenza, Human, medicine, Animals, 030212 general & internal medicine, Immunodiagnostics, Liposome, Hemagglutination assay, biology, General Veterinary, General Immunology and Microbiology, Chemistry, Vaccination, Public Health, Environmental and Occupational Health, Hemagglutination Inhibition Tests, biology.organism_classification, Virology, Rats, Titer, Infectious Diseases, Influenza Vaccines, Quillaja, Molecular Medicine, Adjuvant
الوصف: This work introduces VaxiPatch, a novel vaccination system comprised of subunit glycoprotein vaccine antigens, adjuvants and dermal delivery. For this study, rHA of influenza virus B/Colorado/06/2017 was incorporated into synthetic virosomes, and adjuvant liposomes were formed with QS-21 from Saponaria quillaja, with or without the synthetic TLR4 agonist 3D - (6-acyl) PHAD. These components were concentrated and co-formulated into trehalose with dye. Dermal delivery was achieved using an economical 37-point stainless steel microneedle array, designed for automated fill/finish by microfluidic dispensers used for mass production of immunodiagnostics. Vaccine and adjuvant are deposited to form a sugar glass in a pocket on the side of each of the tips, allowing skin penetration to be performed directly by the rigid steel structure. In this study, Sprague Dawley rats (n = 6 per group) were vaccinated by VaxiPatches containing 0.3 µg of rHA, 0.5 µg QS-21 and 0.2 µg 3D - (6-acyl) PHAD and dye, resulting in antigen-specific IgG titers 100-fold higher than 4.5 µg of FluBlok (p = 0.001) delivered intramuscularly. Similarly, hemagglutination inhibition titers in these animals were 14-fold higher than FluBlok controls (p = 0.01). Non-adjuvanted VaxiPatches were also compared with rHA virosomes injected intramuscularly. Accelerated shelf life studies further suggest that formulated virosomal antigens retain activity for at least two months at 60° C. Further, co-formulation of a dye could provide a visible verification of delivery based on the temporary pattern on the skin. A room-temperature-stable vaccination kit such as VaxiPatch has the potential to increase vaccine use and compliance globally.
تدمد: 1873-2518
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c3f66cf41afdaf5393ee51b34e58e05
https://pubmed.ncbi.nlm.nih.gov/32741668
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....8c3f66cf41afdaf5393ee51b34e58e05
قاعدة البيانات: OpenAIRE
الوصف
تدمد:18732518