Novel Noninvasive Assessment of Microvascular Structure and Function in Humans
| العنوان: | Novel Noninvasive Assessment of Microvascular Structure and Function in Humans |
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| المؤلفون: | Hamish McKirdy, Robert A. McLaughlin, Daniel J. Green, Louise H. Naylor, Andrew Haynes, Kurt J. Smith, Rodney W. Kirk, Howard H. Carter, Raden Argarini, Bryden C. Quirk |
| المصدر: | Medicine & Science in Sports & Exercise. 51:1558-1565 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Erythrocytes, Materials science, genetic structures, Microcirculation, Reproducibility of Results, Physical Therapy, Sports Therapy and Rehabilitation, 030229 sport sciences, Laser Doppler velocimetry, eye diseases, Structure and function, Arterioles, Forearm, 03 medical and health sciences, 0302 clinical medicine, Optical imaging, In vivo, Humans, Orthopedics and Sports Medicine, sense organs, Tomography, Imaging technique, Blood Flow Velocity, Tomography, Optical Coherence, Skin, Biomedical engineering |
| الوصف: | Optical coherence tomography (OCT) is a novel high-resolution imaging technique capable of visualizing in vivo structures at a resolution of ~10 μm. We have developed specialized OCT-based approaches that quantify diameter, speed, and flow rate in human cutaneous microvessels. In this study, we hypothesized that OCT-based microvascular assessments would possess comparable levels of reliability when compared with those derived using conventional laser Doppler flowmetry (LDF).Speckle decorrelation images (OCT) and red blood cell flux (LDF) measures were collected from adjacent forearm skin locations on 2 d (48 h apart), at baseline, and after a 30-min rapid local heating protocol (30°C-44°C) in eight healthy young individuals. OCT postprocessing quantified cutaneous microvascular diameter, speed, flow rate, and density (vessel recruitment) within a region of interest, and data were compared between days.Forearm skin LDF (13 ± 4 to 182 ± 31 AU, P0.05) and OCT-derived diameter (41.8 ± 6.6 vs 64.5 ± 6.9 μm), speed (68.4 ± 9.5 vs 89.0 ± 7.3 μm·s), flow rate (145.0 ± 60.6 vs 485 ± 132 pL·s), and density (9.9% ± 4.9% vs 45.4% ± 5.9%) increased in response to local heating. The average OCT-derived microvascular flow response (pL·s) to heating (234% increase) was lower (P0.05) than the LDF-derived change (AU) (1360% increase). Pearson correlation was significant for between-day local heating responses in terms of OCT flow (r = 0.93, P0.01), but not LDF (P = 0.49). Bland-Altman analysis revealed that between-day baseline OCT-derived flow rates were less variable than LDF-derived flux.Our findings indicate that OCT, which directly visualizes human microvessels, not only allows microvascular quantification of diameter, speed, flow rate, and vessel recruitment but also provides outputs that are highly reproducible. OCT is a promising novel approach that enables a comprehensive assessment of cutaneous microvascular structure and function in humans. |
| تدمد: | 1530-0315 0195-9131 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c73a47642bf45ec9987f4dabdaf9dc1 https://doi.org/10.1249/mss.0000000000001898 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8c73a47642bf45ec9987f4dabdaf9dc1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15300315 01959131 |
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