Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila
| العنوان: | Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila |
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| المؤلفون: | Daniel P. Kiehart, Janice M. Crawford, Glenn S. Edwards, Roger S. Zou, Adrienne R. Wells, U. Serdar Tulu, Adam Sokolow |
| المصدر: | Molecular Biology of the Cell |
| بيانات النشر: | The American Society for Cell Biology, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | animal structures, Embryo, Nonmammalian, genetic structures, Closure (topology), Embryonic Development, Model system, Dissection (medical), Laser Capture Microdissection, Biology, Mechanotransduction, Cellular, Serous Membrane, Extant taxon, medicine, Morphogenesis, Animals, Canthus, Molecular Biology, Cytoskeleton, fungi, Animal Structures, Epithelial Cells, Cell Biology, Anatomy, Articles, Actomyosin, medicine.disease, musculoskeletal system, Dorsal closure, Biomechanical Phenomena, body regions, Drosophila melanogaster, embryonic structures, Epidermis |
| الوصف: | Laser microsurgery and computer tracking of embryo structures indicate that the morphogenetic process of Drosophila dorsal closure requires only forces generated by the amnioserosa tissue. Forces generated by both “zipping” of epidermal tissue at the canthi corners and the resulting actomyosin purse string curvature are not necessary for closure. Drosophila's dorsal closure provides an excellent model system with which to analyze biomechanical processes during morphogenesis. During native closure, the amnioserosa, flanked by two lateral epidermal sheets, forms an eye-shaped opening with canthi at each corner. The dynamics of amnioserosa cells and actomyosin purse strings in the leading edges of epidermal cells promote closure, whereas the bulk of the lateral epidermis opposes closure. Canthi maintain purse string curvature (necessary for their dorsalward forces), and zipping at the canthi shortens leading edges, ensuring a continuous epithelium at closure completion. We investigated the requirement for intact canthi during closure with laser dissection approaches. Dissection of one or both canthi resulted in tissue recoil and flattening of each purse string. After recoil and a temporary pause, closure resumed at approximately native rates until slowing near the completion of closure. Thus the amnioserosa alone can drive closure after dissection of one or both canthi, requiring neither substantial purse string curvature nor zipping during the bulk of closure. How the embryo coordinates multiple, large forces (each of which is orders of magnitude greater than the net force) during native closure and is also resilient to multiple perturbations are key extant questions. |
| اللغة: | English |
| تدمد: | 1939-4586 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ccbcefc3f78784ec3a8a17bcc4b17b0 http://europepmc.org/articles/PMC4230616 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8ccbcefc3f78784ec3a8a17bcc4b17b0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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