TmToll-7 Plays a Crucial Role in Innate Immune Responses Against Gram-Negative Bacteria by Regulating 5 AMP Genes in Tenebrio molitor
| العنوان: | TmToll-7 Plays a Crucial Role in Innate Immune Responses Against Gram-Negative Bacteria by Regulating 5 AMP Genes in Tenebrio molitor |
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| المؤلفون: | In Seok Bang, Yu Jung Kim, Yong Hun Jo, Young Min Bae, Bobae Kim, Chang Eun Kim, Yeon Soo Han, Hye Jin Ko, Soyi Park, Yongseok Lee, Ki Beom Park, Sung Ah Jun |
| المصدر: | Frontiers in Immunology Frontiers in Immunology, Vol 10 (2019) |
| بيانات النشر: | Frontiers Media S.A., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Immunologic diseases. Allergy, Staphylococcus aureus, Gram-negative bacteria, Antimicrobial peptides, Immunology, Gene Expression, medicine.disease_cause, Microbiology, 03 medical and health sciences, antimicrobial peptides, 0302 clinical medicine, Immune system, Hemolymph, Candida albicans, Gram-Negative Bacteria, medicine, Escherichia coli, Immunology and Allergy, Animals, Amino Acid Sequence, Tenebrio, Original Research, Tenebrio molitor, Gene knockdown, Innate immune system, biology, Base Sequence, fungi, biology.organism_classification, Immunity, Innate, 030104 developmental biology, Toll-7, Toll-Like Receptor 7, RNAi, Larva, Insect Proteins, microbial infection, lcsh:RC581-607, AMP assay, 030215 immunology |
| الوصف: | Although it is known that the Drosophila Toll-7 receptor plays a critical role in antiviral autophagy, its function in other insects has not yet been reported. Here, we have identified a Toll-like receptor 7 gene, TmToll-7, in the coleopteran insect T. molitor and examined its potential role in antibacterial and antifungal immunity. We showed that TmToll-7 expression was significantly induced in larvae 6 h after infection with Escherichia coli and Staphylococcus aureus and 9 h after infection with Candida albicans. However, even though TmToll-7 was induced by all three pathogens, we found that TmToll-7 knockdown significantly reduced larval survival to E. coli, but not to S. aureus, and C. albicans infections. To understand the reasons for this difference, we examined the effects of TmToll-7 knockdown on antimicrobial peptide (AMP) gene expression and found a significant reduction of E. coli-induced expression of AMP genes such as TmTenecin-1, TmDefensin-1, TmDefensin-2, TmColeoptericin-1, and TmAttacin-2. Furthermore, TmToll-7 knockdown larvae infected with E. coli showed significantly higher bacterial growth in the hemolymph compared to control larvae treated with Vermilion dsRNA. Taken together, our results suggest that TmToll-7 plays an important role in regulating the immune response of T. molitor to E. coli. |
| اللغة: | English |
| تدمد: | 1664-3224 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ccdbba87cc9b822b569d5a8f05d186f http://europepmc.org/articles/PMC6424196 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8ccdbba87cc9b822b569d5a8f05d186f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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