Dietary yeast-derived mannan oligosaccharides have immune-modulatory properties but do not improve high fat diet-induced obesity and glucose intolerance
| العنوان: | Dietary yeast-derived mannan oligosaccharides have immune-modulatory properties but do not improve high fat diet-induced obesity and glucose intolerance |
|---|---|
| المؤلفون: | Amanda C. M. Pronk, Lisa R. Hoving, Bruno Guigas, Vanessa van Harmelen, Hendrik J. P. van der Zande, Ko Willems van Dijk |
| المصدر: | PLoS ONE PLoS ONE, Vol 13, Iss 5, p e0196165 (2018) PLoS ONE, 13(5) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Physiology, lcsh:Medicine, Adipose tissue, Oligosaccharides, Cell Count, White adipose tissue, Pathology and Laboratory Medicine, Biochemistry, Monocytes, Mannans, Fats, Mice, White Blood Cells, Animal Cells, Medicine and Health Sciences, Glucose homeostasis, lcsh:Science, Immune Response, Mannan, Multidisciplinary, medicine.diagnostic_test, Chemistry, T Cells, Lipids, Liver, Physiological Parameters, medicine.symptom, Cellular Types, Research Article, medicine.medical_specialty, Adipose Tissue, White, Immune Cells, Immunology, Inflammation, Saccharomyces cerevisiae, Diet, High-Fat, Flow cytometry, 03 medical and health sciences, Immune system, Signs and Symptoms, Diagnostic Medicine, Internal medicine, Glucose Intolerance, medicine, Animals, Immunologic Factors, Obesity, Nutrition, Blood Cells, Macrophages, lcsh:R, Body Weight, Biology and Life Sciences, Cell Biology, medicine.disease, Diet, Eosinophils, Mice, Inbred C57BL, 030104 developmental biology, Endocrinology, Dietary Supplements, lcsh:Q |
| الوصف: | The indigestible mannan oligosaccharides (MOS) derived from the outer cell wall of yeast Saccharomyces cerevisiae have shown potential to reduce inflammation. Since inflammation is one of the underlying mechanisms involved in the development of obesity-associated metabolic dysfunctions, we aimed to determine the effect of dietary supplementation with MOS on inflammation and metabolic homeostasis in lean and diet-induced obese mice. Male C57BL/6 mice were fed either a low fat diet (LFD) or a high fat diet (HFD) with, respectively, 10% or 45% energy derived from lard fat, with or without 1% MOS for 17 weeks. Body weight and composition were measured throughout the study. After 12 weeks of intervention, whole-body glucose tolerance was assessed and in week 17 immune cell composition was determined in mesenteric white adipose tissue (mWAT) and liver by flow cytometry and RT-qPCR. In LFD-fed mice, MOS supplementation induced a significant increase in the abundance of macrophages and eosinophils in mWAT. A similar trend was observed in hepatic macrophages. Although HFD feeding induced a classical shift from the anti-inflammatory M2-like macrophages towards the pro-inflammatory M1-like macrophages in both mWAT and liver from control mice, MOS supplementation had no effect on this obesity-driven immune response. Finally, MOS supplementation did not improve whole-body glucose homeostasis in both lean and obese mice.Altogether, our data showed that MOS had extra-intestinal immune modulatory properties in mWAT and liver. However these effects were not substantial enough to significantly ameliorate HFD-induced glucose intolerance or inflammation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d4591a8482bccacfa5b5b62711f708b https://hdl.handle.net/1887/77216 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8d4591a8482bccacfa5b5b62711f708b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |