Low-Level Organic Solvents Improve Multienzyme Whole-Cell Catalytic Synthesis of Myricetin-7-O-Glucuronide
| العنوان: | Low-Level Organic Solvents Improve Multienzyme Whole-Cell Catalytic Synthesis of Myricetin-7-O-Glucuronide |
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| المؤلفون: | Wei Wang, Yun-Song Cao, Yan Yang, Changkun Li, Min-Zhi Liu |
| المصدر: | Catalysts Volume 9 Issue 11 Catalysts, Vol 9, Iss 11, p 970 (2019) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.disease_cause, lcsh:Chemical technology, 01 natural sciences, Catalysis, Cell membrane, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, medicine, Extracellular, lcsh:TP1-1185, Viability assay, Physical and Theoretical Chemistry, organic solvents, Escherichia coli, 010405 organic chemistry, Chemistry, flavonoid glucuronides, engineered Escherichia coli, Combinatorial chemistry, 0104 chemical sciences, 030104 developmental biology, medicine.anatomical_structure, lcsh:QD1-999, Biocatalysis, multienzyme whole-cell biocatalyst, Myricetin, Glucuronide |
| الوصف: | Multienzyme whole-cell biocatalysts are preferred in industrial applications, and two major concerns regarding the use of these biocatalysts, cell viability and cell membrane integrity, must be addressed. In this work, the transformation of myricetin to myricetin-7-O-glucuronide catalyzed by an engineered Escherichia coli strain was taken as the model reaction to examine the impacts of low-level organic solvents on whole-cell biocatalysis. Low-level organic solvents (2%, v/v) showed a significant increase (roughly 13-fold) in myricetin-7-O-glucuronide yields. No obvious compromises of cellular viability and integrity were observed by a flow cytometry assay or in the determination of extracellular protein leakage, suggesting the addition of low-level organic solvents accommodates whole E. coli cells. Furthermore, a scaled-up reaction was conducted to test the capability and efficiency of whole-cell catalysis in the presence of organic solvents. This study presents a promising and simple means to enhance the productivity of multienzyme whole-cell catalysis without losing the barrier functions of the cell membrane. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4344 |
| DOI: | 10.3390/catal9110970 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dd35eb49e9e9b4139259be17f22cb1a |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8dd35eb49e9e9b4139259be17f22cb1a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734344 |
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| DOI: | 10.3390/catal9110970 |