Adaptor protein XB130 regulates the aggressiveness of cholangiocarcinoma
| العنوان: | Adaptor protein XB130 regulates the aggressiveness of cholangiocarcinoma |
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| المؤلفون: | Banchob Sripa, Piti Ungarreevittaya, Chadamas Sakonsinsiri, Raynoo Thanan, Chawalit Pairojkul, Prakasit Sa Ngiamwibool, Sitthichai Iamsaard, Pirawan Poosekeaw |
| المصدر: | PLoS ONE PLoS ONE, Vol 16, Iss 11 (2021) PLoS ONE, Vol 16, Iss 11, p e0259075 (2021) |
| بيانات النشر: | Public Library of Science, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Biochemistry, Metastasis, Cholangiocarcinoma, Phosphatidylinositol 3-Kinases, Cell Movement, Basic Cancer Research, Medicine and Health Sciences, Morphogenesis, XB130, Cell Line, Transformed, Multidisciplinary, Sexual Differentiation, Signal transducing adaptor protein, Small interfering RNA, Middle Aged, Nucleic acids, Cell Motility, Oncology, Cell Processes, Gene Knockdown Techniques, Lymphatic Metastasis, Medicine, Female, Anatomy, Research Article, Signal Transduction, Adult, Histology, Science, Cell Migration, Biology, Transfection, Cholangiocyte, Lymphatic System, Malignant Tumors, Cell Line, Tumor, Genetics, Biomarkers, Tumor, Gene silencing, Humans, Neoplasm Invasiveness, Non-coding RNA, PI3K/AKT/mTOR pathway, Cell Proliferation, Adaptor Proteins, Signal Transducing, Aged, Neoplasm Staging, Cell growth, Biology and Life Sciences, Cancers and Neoplasms, Cell Biology, Gene regulation, Bile Duct Neoplasms, Cell culture, Cancer cell, Cancer research, RNA, Lymph Nodes, Gene expression, Proto-Oncogene Proteins c-akt, Developmental Biology |
| الوصف: | Cholangiocarcinoma (CCA) is a group of heterogenous malignancies arising from bile duct epithelium with distinct pathological features. Adaptor proteins have implicated in cell proliferation, migration, and invasion of different cancer cells. The objective of this study was to assess whether the adaptor protein XB130 (AFAP1L2) is a critical biological determinant of CCA outcome. XB130 expression levels were investigated in four CCA cell lines compared to an immortalized cholangiocyte cell line by Western blotting. Small interfering (si) RNA-mediated XB130 gene silencing was conducted to evaluate the effects of reduced XB130 expression on cell proliferation, migration, and invasion by MTT, transwell migration and cell invasion assay. The immunohistochemical quantification of XB130 levels were performed in surgically resected formalin-fixed, paraffin-embedded specimens obtained from 151 CCA patients. The relationship between XB130 expression and the clinicopathological parameters of CCA patients were analyzed. Our results showed that XB130 was highly expressed in KKU-213A cell line. Knockdown of XB130 using siRNA significantly decreased the proliferation, migration, and invasion properties of KKU-213A cells through the inhibition of PI3K/Akt pathway, suggesting that XB130 plays an important role in CCA progression. Moreover, elevated XB130 expression levels were positive relationship with lymphovascular space invasion (LVSI), intrahepatic type of CCA, high TNM staging (stage III, IV), high T classification (T3, T4), and lymph node metastasis. We provide the first evidence that the overexpression of XB130 is associated with tumorigenic properties of CCA cells, leading to CCA progression with aggressive clinical outcomes. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e3ed6aac68db821a10eedb0a146fb7e http://europepmc.org/articles/PMC8592414 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8e3ed6aac68db821a10eedb0a146fb7e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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