Evaluation of the Antimicrobial Activity of Cationic Polymers against Mycobacteria: Toward Antitubercular Macromolecules
| العنوان: | Evaluation of the Antimicrobial Activity of Cationic Polymers against Mycobacteria: Toward Antitubercular Macromolecules |
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| المؤلفون: | Alasdair T. M. Hubbard, Collette S. Guy, Matthew I. Gibson, Daniel J. Phillips, Elizabeth Fullam, Ian Hands-Portman, James Harrison, Daniel E. Mitchell, Sarah-Jane Richards, Esther Tichauer |
| المصدر: | Biomacromolecules |
| بيانات النشر: | American Chemical Society, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Erythrocytes, Polymers and Plastics, medicine.drug_class, Antitubercular Agents, Bioengineering, 02 engineering and technology, Drug resistance, 010402 general chemistry, Methacrylate, Antimycobacterial, 01 natural sciences, Hemolysis, Article, Microbiology, Mycobacterium, Biomaterials, Mycobacterium tuberculosis, Materials Chemistry, medicine, Polyamines, biology, Chemistry, Cell Membrane, Cationic polymerization, 021001 nanoscience & nanotechnology, Antimicrobial, biology.organism_classification, Polyelectrolytes, 0104 chemical sciences, 3. Good health, Nylons, Biochemistry, Methacrylates, 0210 nano-technology, Antibacterial activity, Bacteria, RC |
| الوصف: | Antimicrobial resistance is a global healthcare problem with a dwindling arsenal of usable drugs. Tuberculosis, caused by Mycobacterium tuberculosis, requires long-term combination therapy and multi- and totally drug resistant strains have emerged. This study reports the antibacterial activity of cationic polymers against mycobacteria, which are distinguished from other Gram-positive bacteria by their unique cell wall comprising a covalently linked mycolic acid−arabinogalactan−peptidoglycan complex (mAGP), interspersed with additional complex lipids which helps them persist in their host. The present study finds that poly(dimethylaminoethyl methacrylate) has particularly potent antimycobacterial activity and high selectivity over two Gram-negative strains. Removal of the backbone methyl group (poly(dimethylaminoethyl acrylate)) decreased antimycobacterial activity, and poly(aminoethyl methacrylate) also had no activity against mycobacteria. Hemolysis assays revealed poly(dimethylaminoethyl methacrylate) did not disrupt red blood cell membranes. Interestingly, poly(dimethylaminoethyl methacrylate) was not found to permeabilize mycobacterial membranes, as judged by dye exclusion assays, suggesting the mode of action is not simple membrane disruption, supported by electron microscopy analysis. These results demonstrate that synthetic polycations, with the correctly tuned structure are useful tools against mycobacterial infections, for which new drugs are urgently required. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1526-4602 1525-7797 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e9a842a5bdbc5f3144ec776659e6d9c http://europepmc.org/articles/PMC5435458 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8e9a842a5bdbc5f3144ec776659e6d9c |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15264602 15257797 |
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