Characterization of [3H]Ro 16-6491 binding to digitonin solubilized monoamine oxidase-B and purification of the enzyme from human platelets by affinity chromatography
التفاصيل البيبلوغرافية
العنوان:
Characterization of [3H]Ro 16-6491 binding to digitonin solubilized monoamine oxidase-B and purification of the enzyme from human platelets by affinity chromatography
In the present paper we describe the use of digitonin as a suitable detergent for the effective solubilization of MAO-B from human platelets and frontal cortex membrane preparations and the characteristics of the solubilized enzyme using the selective MAO-B inhibitor [ 3 H] Ro 16-6491 [N-(2-aminoethyl)-p-chlorobenzamide HCl] as binding probe. In addition, we describe an affinity chromatography method for human platelet MAO-B, using as affinity ligand a derivative of Ro 19-6327 [N-(2-aminoethyl)-5-chloro-2-pyridine carboxamide HCl], a compound in clinical trial belonging to a novel class of reversible and selective MAO-B inhibitors