Joint Degradation in a Monkey Model of Collagen-Induced Arthritis: Role of Cathepsin K Based on Biochemical Markers and Histological Evaluation
| العنوان: | Joint Degradation in a Monkey Model of Collagen-Induced Arthritis: Role of Cathepsin K Based on Biochemical Markers and Histological Evaluation |
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| المؤلفون: | Hiroyuki Yamada, Yasuo Ochi, Minqi Li, Satoshi Nishikawa, Makoto Tanaka, Norio Amizuka, Naoto Horai, Hiroshi Mori |
| المصدر: | International Journal of Rheumatology, Vol 2016 (2016) International Journal of Rheumatology |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, musculoskeletal diseases, medicine.medical_specialty, Deoxypyridinoline, Pathology, lcsh:Diseases of the musculoskeletal system, Article Subject, Immunology, Type II collagen, Arthritis, Bone resorption, 03 medical and health sciences, chemistry.chemical_compound, Rheumatology, Internal medicine, medicine, Cathepsin K, Cathepsin, business.industry, Cartilage, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, chemistry, lcsh:RC925-935, business, Type I collagen, Research Article |
| الوصف: | The role of cathepsin K in joint degradation in a model of collagen-induced arthritis (CIA) in cynomolgus monkey was examined using biochemical markers and histology. Joint swelling, urinary C-telopeptide of type II collagen (CTX-II), deoxypyridinoline (DPD), and N- and C-telopeptides of type I collagen (NTX and CTX-I, resp.) were analyzed. Immunohistochemistry of type II collagen, cathepsin K, and CTX-II were performed using joints. Joint swelling reached peak on day 42 and continued at this level. The CTX-II level peaked on day 28 and declined thereafter, while CTX-I, NTX, and DPD reached plateau on day 43. Joint swelling was positively correlated with CTX-II increases on days 20 and 42/43, with increases in CTX-I and NTX/Cr on days 42/43 and 84, and with DPD increases throughout the study period. Intense cathepsin K staining was observed in osteoclasts and in articular cartilage and synovial tissue in arthritic joints. CTX-II was present in the superficial layer of articular cartilage in CIA monkeys. Evidence from biochemical markers suggests that matrix degradation in the CIA model starts with degradation of cartilage, rather than bone resorption. Cathepsin K expressed in osteoclasts, articular cartilage, and synovial tissue may contribute to degradation of cartilage. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1687-9279 1687-9260 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::902e0d71a9f41911164fd0e51c1ea8d4 https://doaj.org/article/2d44ceccbb7b43ff82b22b1296e8d8bc |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....902e0d71a9f41911164fd0e51c1ea8d4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16879279 16879260 |
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