Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner
| العنوان: | Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner |
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| المؤلفون: | Joshua D. W. Teo, Zhaona Wu, Kevin S. W. Tan, Haris Mirza |
| المصدر: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| بيانات النشر: | Hindawi Limited, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cell Membrane Permeability, Article Subject, Enterocyte, lcsh:Medicine, Apoptosis, Caspase 3, General Biochemistry, Genetics and Molecular Biology, Cell Line, Host-Parasite Interactions, medicine, Humans, Intestinal Mucosa, Fragmentation (cell biology), Cytoskeleton, Caspase, Cell Nucleus, Blastocystis, General Immunology and Microbiology, biology, Tight junction, lcsh:R, General Medicine, biology.organism_classification, Cell biology, Enterocytes, medicine.anatomical_structure, Caspases, Zonula Occludens-1 Protein, biology.protein, Caco-2 Cells, Research Article |
| الوصف: | Blastocystisis an emerging protistan parasite colonizing the human intestine. It is frequently reported to cause general intestinal symptoms of vomiting, diarrhea, and abdominal pain. We recently demonstrated thatBlastocystisrearranged cytoskeletal proteins and induced intestinal epithelial barrier compromise. The effect ofBlastocystison enterocyte apoptosis is unknown, and a possible link between microbially induced enterocyte apoptosis and increased epithelial permeability has yet to be determined. The aim of this study is to assess ifBlastocystisinduces human enterocyte apoptosis and whether this effect influences human intestinal epithelial barrier function. Monolayers of polarized human colonic epithelial cell-line Caco-2 were incubated withBlastocystissubtype 7 and subtype 4. Assays for both early and late markers of apoptosis, phosphatidylserine externalization, and nuclear fragmentation, respectively, showed thatBlastocystisST-7, but not ST-4, significantly increased apoptosis in enterocytes, suggesting thatBlastocystisexhibits host specificity and strain-to-strain variation in pathogenicity. ST-7 also activated Caco-2 caspases 3 and 9 but not 8. ST-7 induced changes in epithelial resistance, permeability, and tight junction (ZO-1) localization. Pretreatment of Caco-2 monolayers with a pan-caspase inhibitor z-VAD-fmk significantly inhibited these changes. This suggests a role for enterocyte apoptosis inBlastocystis-mediated epithelial barrier compromise in the human intestine. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::905142d58a01020bfbdcdf5a43b8f719 https://doaj.org/article/2cc64a79273143de8f45729c26a3c880 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....905142d58a01020bfbdcdf5a43b8f719 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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