Retinal Cell Type DNA Methylation and Histone Modifications Predict Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures
| العنوان: | Retinal Cell Type DNA Methylation and Histone Modifications Predict Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures |
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| المؤلفون: | Daniel J. Hiler, Issam Aldiri, Marc Valentine, Abbas Shirinifard, Michael A. Dyer, Suresh Thiagarajan, Xin Zhou, Dianna A. Johnson, Xiang Chen, Jiakun Zhang, Lu Wang, Lyra Griffiths, Andras Sablauer, Marie E. Barabas, Beisi Xu, Sharon Frase |
| المصدر: | Cell Reports, Vol 22, Iss 10, Pp 2601-2614 (2018) Cell reports |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cell type, Organogenesis, Induced Pluripotent Stem Cells, Cell Culture Techniques, Article, Retina, General Biochemistry, Genetics and Molecular Biology, Epigenesis, Genetic, Histones, Kruppel-Like Factor 4, Mice, 03 medical and health sciences, Animals, Epigenetics, Promoter Regions, Genetic, Induced pluripotent stem cell, lcsh:QH301-705.5, Cell Nucleus, biology, Cell Differentiation, DNA Methylation, Cellular Reprogramming, Cell biology, Organoids, Enhancer Elements, Genetic, 030104 developmental biology, Histone, lcsh:Biology (General), KLF4, DNA methylation, biology.protein, Ectopic expression, Protein Processing, Post-Translational, Reprogramming, Biomarkers |
| الوصف: | SUMMARY Diverse cell types can be reprogrammed into pluripotent stem cells by ectopic expression of Oct4 (Pou5f1), Klf4, Sox3, and Myc. Many of these induced pluripotent stem cells (iPSCs) retain memory, in terms of DNA methylation and histone modifications (epigenetic memory), of their cellular origins, and this may bias subsequent differentiation. Neurons are difficult to reprogram, and there has not been a systematic side-by-side characterization of reprogramming efficiency or epigenetic memory across different neuronal subtypes. Here, we compare reprogramming efficiency of five different retinal cell types at two different stages of development. Retinal differentiation from each iPSC line was measured using a quantitative standardized scoring system called STEM-RET and compared to the epigenetic memory. Neurons with the lowest reprogramming efficiency produced iPSC lines with the best retinal differentiation and were more likely to retain epigenetic memory of their cellular origins. In addition, we identified biomarkers of iPSCs that are predictive of retinal differentiation. Graphical abstract |
| تدمد: | 2211-1247 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9052c6d8edb1f74f1ada0d214421852d https://doi.org/10.1016/j.celrep.2018.01.075 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9052c6d8edb1f74f1ada0d214421852d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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