Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements
| العنوان: | Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements |
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| المؤلفون: | B. Achour, Walid-Sabri Hamadou, Marie-Laure Boulland, Thierry Fest, H. Regaieg, Abderrahim Khelif, Yosra Ben Youssef, Zohra Soua, Sawsen Besbes |
| المساهمون: | Université de Sousse, Microenvironment, Cell Differentiation, Immunology and Cancer (MICMAC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CHU Pontchaillou [Rennes], MESRS, Ministére de lEnseignement Supérieur et de la Recherche Scientifique, Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| المصدر: | Brazilian Journal of Medical and Biological Research, Volume: 50, Issue: 1, Article number: e5426, Published: 16 JAN 2017 Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Associação Brasileira de Divulgação Científica, 2017, 50 (1), pp.e5426. ⟨10.1590/1414-431X20165426⟩ Brazilian Journal of Medical and Biological Research, Vol 50, Iss 1 Brazilian Journal of Medical and Biological Research v.50 n.1 2017 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, 2017, 50 (1), pp.e5426. ⟨10.1590/1414-431X20165426⟩ |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, IGK gene, Neoplasm, Residual, Lineage (genetic), Adolescent, Physiology, IGH gene, Immunology, Biophysics, Ocean Engineering, [SDV.CAN]Life Sciences [q-bio]/Cancer, B-ALL minimal residual disease (MRD), Biology, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Biochemistry, 03 medical and health sciences, 0302 clinical medicine, hemic and lymphatic diseases, Multiplex polymerase chain reaction, Biomarkers, Tumor, Humans, Clinical Investigation, B Acute Lymphoblastic Leukemia, General Pharmacology, Toxicology and Pharmaceutics, Gene, lcsh:QH301-705.5, Gene Rearrangement, lcsh:R5-920, General Neuroscience, Induction chemotherapy, Cell Biology, General Medicine, Middle Aged, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Minimal residual disease, Molecular biology, 3. Good health, Real-time polymerase chain reaction, lcsh:Biology (General), Child, Preschool, 030220 oncology & carcinogenesis, RQ-PCR, Cancer research, Female, Immunoglobulin Gene Rearrangement, lcsh:Medicine (General), 030215 immunology |
| الوصف: | International audience; IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven B-ALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of B-ALL, introduced for the first time in Tunisian laboratories. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 0100-879X 0034-7310 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9062be53dbdf0464b5af0fd1893e4adf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100703&lng=en&tlng=en |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9062be53dbdf0464b5af0fd1893e4adf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0100879X 00347310 |
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