Propoxur resistance associated with insensitivity of acetylcholinesterase (AChE) in the housefly, Musca domestica (Diptera: Muscidae)
| العنوان: | Propoxur resistance associated with insensitivity of acetylcholinesterase (AChE) in the housefly, Musca domestica (Diptera: Muscidae) |
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| المؤلفون: | Juanjuan Xin, Yi Zhang, Xiao-peng Zeng, Zhuo Ma, Jing Li, Xiwu Gao, Chunmei You, Chao Shan |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-7 (2020) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Insecticides, Science, Propoxur, Polymorphism, Single Nucleotide, 01 natural sciences, Article, Gene Expression Regulation, Enzymologic, Insecticide Resistance, 03 medical and health sciences, chemistry.chemical_compound, Houseflies, Complementary DNA, Phoxim, Azamethiphos, Animals, Housefly, Multidisciplinary, biology, biology.organism_classification, Molecular biology, Acetylcholinesterase, Enzymes, 010602 entomology, 030104 developmental biology, Deltamethrin, chemistry, Muscidae, Mutation, Insect Proteins, Medicine, Cholinesterase Inhibitors, Entomology |
| الوصف: | Two unique housefly strains, PSS and N-PRS (near-isogenic line with the PSS), were used to clarify the mechanisms associated with propoxur resistance in the housefly, Musca domestica. The propoxur-selected resistant (N-PRS) strain exhibited >1035-fold resistance to propoxur and 1.70-, 12.06-, 4.28-, 57.76-, and 57.54-fold cross-resistance to beta-cypermethrin, deltamethrin, bifenthrin, phoxim, and azamethiphos, respectively, compared to the susceptible (PSS) strain. We purified acetylcholinesterase (AChE) from the N-PRS and PSS strains using a procainamide affinity column and characterized the AChE. The sensitivity of AChE to propoxur based on the bimolecular rate constant (Ki) was approximately 100-fold higher in the PSS strain compared to the N-PRS strain. The cDNA encoding Mdace from both the N-PRS strain and the PSS strain were cloned and sequenced using RT-PCR. The cDNA was 2073 nucleotides long and encoded a protein of 691 amino acids. A total of four single nucleotide polymorphisms (SNPs), I162M, V260L, G342A, and F407Y, were present in the region of the active site of AChE from the N-PRS strain. The transcription level and DNA copy number of Mdace were significantly higher in the resistant strain than in the susceptible strain. These results indicated that mutations combined with the up-regulation of Mdace might be essential in the housefly resistance to propoxur. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90b061a2c6645b1ed44d3ca2e8127c27 https://doaj.org/article/1176566d813f4712be81ffcdcd31357b |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....90b061a2c6645b1ed44d3ca2e8127c27 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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