Differential Utilization of Dietary Fatty Acids in Benign and Malignant Cells of the Prostate
| العنوان: | Differential Utilization of Dietary Fatty Acids in Benign and Malignant Cells of the Prostate |
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| المؤلفون: | Julia Höfer, Erich Gnaiger, Lukas Kenner, Bernd Schöpf, Bernhard Perktold, Iris E. Eder, Helmut Klocker, Andrea Dueregger, Astrid Aufinger, Reinhold Ramoner, Theresa Eder |
| المصدر: | PLoS ONE, Vol 10, Iss 8, p e0135704 (2015) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Cell Survival, Cellular respiration, Cell Respiration, Gene Dosage, lcsh:Medicine, Ketone Bodies, Biology, Carbohydrate metabolism, DNA, Mitochondrial, Oxidative Phosphorylation, Cell Line, Tumor, LNCaP, Humans, Glycolysis, lcsh:Science, Triglycerides, Aged, chemistry.chemical_classification, Multidisciplinary, Fatty Acids, lcsh:R, Prostate, Prostatic Neoplasms, Fatty acid, Epithelial Cells, Middle Aged, Dietary Fats, Mitochondria, Biochemistry, chemistry, Genome, Mitochondrial, Cancer cell, Ketone bodies, lcsh:Q, Mitochondrial Size, Energy source, Research Article |
| الوصف: | Tumor cells adapt via metabolic reprogramming to meet elevated energy demands due to continuous proliferation, for example by switching to alternative energy sources. Nutrients such as glucose, fatty acids, ketone bodies and amino acids may be utilized as preferred substrates to fulfill increased energy requirements. In this study we investigated the metabolic characteristics of benign and cancer cells of the prostate with respect to their utilization of medium chain (MCTs) and long chain triglycerides (LCTs) under standard and glucose-starved culture conditions by assessing cell viability, glycolytic activity, mitochondrial respiration, the expression of genes encoding key metabolic enzymes as well as mitochondrial mass and mtDNA content. We report that BE prostate cells (RWPE-1) have a higher competence to utilize fatty acids as energy source than PCa cells (LNCaP, ABL, PC3) as shown not only by increased cell viability upon fatty acid supplementation but also by an increased ß-oxidation of fatty acids, although the base-line respiration was 2-fold higher in prostate cancer cells. Moreover, BE RWPE-1 cells were found to compensate for glucose starvation in the presence of fatty acids. Of notice, these findings were confirmed in vivo by showing that PCa tissue has a lower capacity in oxidizing fatty acids than benign prostate. Collectively, these metabolic differences between benign and prostate cancer cells and especially their differential utilization of fatty acids could be exploited to establish novel diagnostic and therapeutic strategies. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91051e3a4fba17db1950b4017498ef99 https://doi.org/10.1371/journal.pone.0135704 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....91051e3a4fba17db1950b4017498ef99 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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