Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy
| العنوان: | Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
|---|---|
| المؤلفون: | Guilherme D. Melo, Marlos Gonçalves Sousa, Aparecido Antonio Camacho, F. A. Rosa, Gustavo Dittrich, F. N. Gava, Gisele Fabrino Machado, Sheila Saraiva da Silva Nogueira |
| المساهمون: | Lyssavirus, épidémiologie et neuropathologie - Lyssavirus Epidemiology and Neuropathology, Institut Pasteur [Paris], Universidade Estadual Paulista (Unesp), Universidade Federal do Paraná (UFPR) |
| المصدر: | Pesquisa Veterinária Brasileira Pesquisa Veterinária Brasileira, Colégio Brasileiro de Patologia Animal-CBPA, 2018, 38 (2), pp.320-327. ⟨10.1590/1678-5150-PVB-4990⟩ Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Pesquisa Veterinária Brasileira v.38 n.2 2018 Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Vol 38, Iss 2, Pp 320-327 |
| بيانات النشر: | FapUNIFESP (SciELO), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | cardiac disease, 0301 basic medicine, Cardiac function curve, rabbits, medicine.medical_specialty, clinics, Cardiomyopathy, Collagenase, Echocardiogram, 030204 cardiovascular system & hematology, Matrix metalloproteinase, doxorubicin, Cardiac disease, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Clinics, Zymography, Doxorubicin, Enzyme activity, lcsh:Veterinary medicine, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, General Veterinary, biology, business.industry, zymography, medicine.disease, echocardiogram, Enzyme assay, enzyme activity, collagenase, Matrix metalloproteinases, 030104 developmental biology, Cardiology, biology.protein, lcsh:SF600-1100, Rabbits, Doxorubicin induced cardiomyopathy, business, cardiomyopathy, medicine.drug |
| الوصف: | International audience; ABSTRACT: Some studies have shown the role played by matrix metalloproteinases and their inhibitors in doxorubicin cardiotoxicity. In this study, we sought to investigate how plasma and myocardial MMP 2 and 9 perform in rabbits with doxorubicin-induced cardiomyopathy, searching for a correlation between the activity of these collagenases and cardiac remodeling. Cardiomyopathy was induced by doxorubicin given intravenously twice a week for six consecutive weeks. Plasma MMP activity and the echocardiogram were assessed at baseline, and at 15 and 45 days after first injection of doxorubicin. The myocardial activity of these enzymes was solely evaluated in nine rabbits at 45 days, and results were compared with nine healthy controls. We only identified the full-length forms of both MMP 2 and 9 throughout the study. The plasma pro-MMP 2 reduced along the deterioration of cardiac function, while the pro-MMP 9 increased significantly at T45 as compared to baseline and T15. A negative significant correlation was found to exist between the plasma activity of pro-MMP 2 and mitral E-to-mitral septal annular early diastolic velocity ratio, which is an estimate of mean left atrial pressure and congestion. Only pro-MMP 2 was found in myocardial samples, and mean activity of such enzyme was statistically lower than that recorded for healthy controls. Although no active form was documented for either collagenase, the duration of the treatment with doxorubicin played a role in the alteration of plasma pro-forms activity. However, these changes could not be associated with most echocardiographic parameters that are supportive of cardiac remodeling.; RESUMO: Alguns estudos já demonstraram o papel exercido pelas metaloproteinases de matriz e seus inibidores na cardiotoxicidade promovida pela doxorrubicina. Assim, este estudo teve como objetivo investigar o comportamento das MMPs 2 e 9 plasmáticas e miocárdicas em coelhos com cardiomiopatia induzida pela doxorrubicina, buscando determinar se há correlação entre a atividade dessas colagenases e o remodelamento cardíaco. A cardiomiopatia foi induzida pela doxorrubicina aplicada por via intravenosa duas vezes por semana ao longo de seis semanas consecutivas. A atividade plasmática das MMPs e o ecocardiograma foram avaliados no momento basal e aos 15 e 45 dias após a primeira aplicação da doxorrubicina. A atividade miocárdica dessas enzimas foi quantificada em apenas nove coelhos aos 45 dias e os resultados comparados com outros nove controles saudáveis. Foram identificadas apenas as formas inativas das MMPs 2 e 9 durante todo o estudo. A pro-MMP 2 plasmática reduziu à medida que a função cardiaca se deteriorou, enquanto a pro-MMP 9 aumentou significativamente em T45 quando comparada aos momentos basal e T15. Houve correlação negativa significativa entre a atividade plasmática da pro-MMP 2 e a relação entre E mitral e a velocidade anular mitral no início da diástole, um parâmetro que permite estimar a pressão atrial esquerda média e a congestão. Apenas a pro-MMP 2 foi documentada nas amostras miocárdicas dos coelhos com cardiomiopatia e atividade media dessa enzima foi estatisticamente menor que aquela observada nos controles saudáveis. Embora a forma ativa de ambas as colagenases não tenha sido identificada, o tempo de tratamento com doxorrubicina interferiu na atividade das formas inativas plasmáticas. Contudo, essas alterações não se associaram com a maioria dos parâmetros ecocardiográficos que indicam remodelamento cardíaco. |
| وصف الملف: | text/html |
| تدمد: | 1678-5150 0100-736X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::915e147574d15f8fe190588a05bcb4cd https://doi.org/10.1590/1678-5150-pvb-4990 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....915e147574d15f8fe190588a05bcb4cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16785150 0100736X |
|---|