Colorimetric Solid-Phase Capture Hybridization Assay for Detection of Amplified Borrelia burgdorferi DNA
| العنوان: | Colorimetric Solid-Phase Capture Hybridization Assay for Detection of Amplified Borrelia burgdorferi DNA |
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| المؤلفون: | A. Van Elsen, Edmond Godfroid, Alex Bollen, Bernard Hoyois, Frederic Mansy, M. J. De Vos |
| المصدر: | BioTechniques. 21:122-125 |
| بيانات النشر: | Future Science Ltd, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | DNA, Bacterial, Biotin, Spirochaetaceae, Polymerase Chain Reaction, Sensitivity and Specificity, General Biochemistry, Genetics and Molecular Biology, law.invention, chemistry.chemical_compound, Borrelia burgdorferi Group, law, Synovial Fluid, Humans, Borrelia burgdorferi, Colorimetry, Polymerase chain reaction, Skin, Lyme Disease, biology, Hybridization probe, Nucleic Acid Hybridization, Templates, Genetic, bacterial infections and mycoses, biology.organism_classification, Molecular biology, chemistry, Agarose gel electrophoresis, Polystyrenes, Lyme disease microbiology, DNA Probes, DNA, Biotechnology |
| الوصف: | To simplify detection procedures of DNA fragments resulting from PCR, we developed a colorimetric microplate hybridization assay. This format was used for the identification of Borrelia burgdorferi sensu lato, the causal agent of Lyme disease. The system relied on the use of a specific capture probe covalently linked to polystyrene plates and a specific polybiotinylated detection probe. DNA fragments, resulting from PCR and sandwiched between these two probes, were detected by enzymatic color development. The new detection format outperformed agarose gel electrophoresis of PCR products in sensitivity and specificity. Moreover, in view of its rapidity and simplicity, the system proved appropriate for the routine diagnostic analysis of clinical specimens from Lyme disease patients. The proposed detection format can be adapted easily to other DNA targets and is suitable for automation. |
| تدمد: | 1940-9818 0736-6205 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::919efbe4afb769c5ad9dff4247d106c7 https://doi.org/10.2144/96211rr03 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....919efbe4afb769c5ad9dff4247d106c7 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 19409818 07366205 |
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