Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens
| العنوان: | Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens |
|---|---|
| المؤلفون: | Timothy J. Powell, Karsten Skjødt, Lawrence G. Hunt, Olli Vainio, Michael V. Wiles, Francis Vilbois, Patricia Riegert, James C. Kaufman, Hans-Joachim Wallny, Jan Salomonsen, D Avila |
| المصدر: | University of Southern Denmark Wallny, H-J, Avila, D, Hunt, L G, Powell, T J, Riegert, P, Salomonsen, J, Skjødt, K, Vainio, O, Vilbois, F, Wiles, M V & Kaufman, J 2006, ' Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens ', Proceedings of the National Academy of Science of the United States of America, vol. 103, no. 5, pp. 1434-1439 . |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Models, Molecular, Sarcoma, Avian, DNA, Complementary, Time Factors, Antigen presentation, Amino Acid Motifs, Molecular Sequence Data, Genes, MHC Class I, Major histocompatibility complex, Avian sarcoma virus, Complementary DNA, MHC class I, Animals, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, Peptide sequence, Poultry Diseases, Genes, Dominant, Genetics, Rous sarcoma virus, Antigen Presentation, Multidisciplinary, biology, Sequence Homology, Amino Acid, Haplotype, Biological Sciences, biology.organism_classification, Flow Cytometry, Molecular biology, Avian Sarcoma Viruses, Haplotypes, biology.protein, Peptides, Chickens |
| الوصف: | Udgivelsesdato: 31 januar Compared with the MHC of typical mammals, the chicken MHC is smaller and simpler, with only two class I genes found in the B12 haplotype. We make five points to show that there is a single-dominantly expressed class I molecule that can have a strong effect on MHC function. First, we find only one cDNA for two MHC haplotypes (B14 and B15) and cDNAs corresponding to two genes for the other six (B2, B4, B6, B12, B19, and B21). Second, we find, for the B4, B12, and B15 haplotypes, that one cDNA is at least 10-fold more abundant than the other. Third, we use 2D gel electrophoresis of class I molecules from pulse-labeled cells to show that there is only one heavy chain spot for the B4 and B15 haplotypes, and one major spot for the B12 haplotype. Fourth, we determine the peptide motifs for B4, B12, and B15 cells in detail, including pool sequences and individual peptides, and show that the motifs are consistent with the peptides binding to models of the class I molecule encoded by the abundant cDNA. Finally, having shown for three haplotypes that there is a single dominantly expressed class I molecule at the level of RNA, protein, and antigenic peptide, we show that the motifs can explain the striking MHC-determined resistance and susceptibility to Rous sarcoma virus. These results are consistent with the concept of a "minimal essential MHC" for chickens, in strong contrast to typical mammals. |
| تدمد: | 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91b3988898f6a41bc51f11622de7d299 https://pubmed.ncbi.nlm.nih.gov/16432226 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....91b3988898f6a41bc51f11622de7d299 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00278424 |
|---|