MRI evidence that glibenclamide reduces acute lesion expansion in a rat model of spinal cord injury
| العنوان: | MRI evidence that glibenclamide reduces acute lesion expansion in a rat model of spinal cord injury |
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| المؤلفون: | Rao P. Gullapalli, Orest Tsymbalyuk, J M Simard, Phillip G. Popovich, Michael Kilbourne, Volodymyr Gerzanich, John Caridi |
| المصدر: | Spinal cord |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Aging, Rat model, Treatment outcome, Sur1-Trpm4 channel, Hemorrhage, Article, Lesion, Glibenclamide, 03 medical and health sciences, 0302 clinical medicine, Text mining, Glyburide, medicine, Animals, Rats, Long-Evans, Spinal cord injury, Spinal Cord Injuries, 030304 developmental biology, 0303 health sciences, progressive hemorrhagic necrosis, medicine.diagnostic_test, business.industry, Magnetic resonance imaging, General Medicine, Long evans, medicine.disease, riluzole, Magnetic Resonance Imaging, spinal cord injury, Rats, Disease Models, Animal, Treatment Outcome, Neurology, glibenclamide, Anesthesia, Female, Neurology (clinical), medicine.symptom, business, 030217 neurology & neurosurgery, medicine.drug, MRI |
| الوصف: | Study design Experimental, controlled, animal study. Objectives To use non-invasive magnetic resonance imaging (MRI) to corroborate invasive studies showing progressive expansion of a hemorrhagic lesion during the early hours after spinal cord trauma and to assess the effect of glibenclamide, which blocks Sur1-Trpm4 channels implicated in post-traumatic capillary fragmentation, on lesion expansion. Setting Baltimore. Methods Adult female Long–Evans rats underwent unilateral impact trauma to the spinal cord at C7, which produced ipsilateral but not contralateral primary hemorrhage. In series 1 (six control rats and six administered glibenclamide), hemorrhagic lesion expansion was characterized using MRI at 1 and 24 h after trauma. In series 2, hemorrhagic lesion size was characterized on coronal tissue sections at 15 min (eight rats) and at 24 h after trauma (eight control rats and eight administered glibenclamide). Results MRI (T2 hypodensity) showed that lesions expanded 2.3±0.33-fold (P0.05) in glibenclamide-treated rats. Measuring the areas of hemorrhagic contusion on tissue sections at the epicenter showed that lesions expanded 2.2±0.12-fold (P0.05) in glibenclamide-treated rats. Glibenclamide treatment was associated with significantly better neurological function (unilateral BBB scores) at 24 h in both the ipsilateral (median scores, 9 vs 0; P |
| اللغة: | English |
| تدمد: | 1476-5624 1362-4393 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91b521fd0fdc877383f428e37250407c http://europepmc.org/articles/PMC4076111 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....91b521fd0fdc877383f428e37250407c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765624 13624393 |
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