The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo
| العنوان: | The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo |
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| المؤلفون: | Willekers, Sven, Tessadori, Federico, van der Vaart, Babet, Henning, Heiko H., Stucchi, Riccardo, Altelaar, Maarten, Roelen, Bernard A.J., Akhmanova, Anna, Bakkers, Jeroen, Sub Cell Biology, Voortplanting paard, Afd Biomol.Mass Spect. and Proteomics, Anatomie en fysiologie, Celbiologie, Al het onderzoek van de faculteit Diergeneeskunde, CS_Cancer |
| المساهمون: | Hubrecht Institute for Developmental Biology and Stem Cell Research, Sub Cell Biology, Voortplanting paard, Afd Biomol.Mass Spect. and Proteomics, Anatomie en fysiologie, Celbiologie, Al het onderzoek van de faculteit Diergeneeskunde, CS_Cancer |
| المصدر: | Development (Cambridge, England), 149(16), 1. Company of Biologists Ltd Development (Cambridge), 149(16). Company of Biologists Ltd |
| بيانات النشر: | The Company of Biologists, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Centrosome, Male, Cell division, Ccdc11, Semen/metabolism, Tubulin/metabolism, Maternal, Microtubule-Organizing Center/metabolism, Cfap53, Paternal, Centrioles/metabolism, Centrosome/metabolism, MTOC, Semen, Tubulin, Zebrafish/metabolism, Centriolar satellites, Animals, Molecular Biology, Microtubule-Organizing Center, Zebrafish, Centrioles, Developmental Biology |
| الوصف: | In embryos of most animal species, the zygotic centrosome is assembled by the centriole derived from the sperm cell and pericentriolar proteins present in the oocyte. This zygotic centrosome acts as a microtubule organizing center (MTOC) to assemble the sperm aster and mitotic spindle. As MTOC formation has been studied mainly in adult cells, very little is known about the formation of the zygotic MTOC. Here, we show that zebrafish (Danio rerio) embryos lacking either maternal or paternal Cfap53, a centriolar satellite protein, arrest during the first cell cycle. Although Cfap53 is dispensable for sperm aster function, it aids proper formation of the mitotic spindle. During cell division, Cfap53 colocalizes with γ-tubulin and with other centrosomal and centriolar satellite proteins at the MTOC. Furthermore, we find that γ-tubulin localization at the MTOC is impaired in the absence of Cfap53. Based on these results, we propose a model in which Cfap53 deposited in the oocyte and the sperm participates in the organization of the zygotic MTOC to allow mitotic spindle formation. |
| وصف الملف: | application/pdf |
| تدمد: | 1477-9129 0950-1991 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9201a33b12009a9e3c67971c904e84a8 https://doi.org/10.1242/dev.198762 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9201a33b12009a9e3c67971c904e84a8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779129 09501991 |
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