The Potential Use of Enamel Matrix Derivative for In Situ Anterior Cruciate Ligament Tissue Engineering: A Translational In Vitro Investigation
| العنوان: | The Potential Use of Enamel Matrix Derivative for In Situ Anterior Cruciate Ligament Tissue Engineering: A Translational In Vitro Investigation |
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| المؤلفون: | Bahaa B. Seedhom, Steven J. Brookes, El Mustafa Raif, Michael P. Messenger |
| المصدر: | Tissue Engineering. 13:2041-2051 |
| بيانات النشر: | Mary Ann Liebert Inc, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Tissue Engineering, Chemistry, Regeneration (biology), Synovial Membrane, Cell Culture Techniques, General Engineering, Anatomy, Cell biology, medicine.anatomical_structure, Dental Enamel Proteins, Synovial Cell, Tissue engineering, Cell culture, Enamel matrix derivative, Cell Adhesion, Ligament, medicine, Animals, Periodontal fiber, Cattle, Anterior Cruciate Ligament, Fibroblast, Cells, Cultured, Cell Proliferation |
| الوصف: | Polyester scaffolds have been used as an alternative to autogenous tissues for the reconstruction of the anterior cruciate ligament (ACL). They are biocompatible and encourage tissue infiltration, leading to neoligament formation. However, rupture can occur, caused by abrasion of the scaffold against the bone tunnels through which it is implanted. Good early tissue induction is therefore considered essential to protect the scaffold from this abrasion. Enamel matrix derivative (EMD) is used clinically in the treatment of periodontal disease. It is a complex mix of proteins with growth factor-like activity, which enhances periodontal ligament fibroblast attachment, proliferation, and differentiation, leading to the regeneration of periodontal bone and ligament tissues. We hypothesized that EMD might, in a similar manner, enhance tissue induction around scaffolds used in ACL reconstruction. This preliminary investigation adopted a translational approach, modelling in vitro 3 possible clinical modes of EMD administration, to ascertain the suitability of each protocol for application in an animal model or clinically. Preliminary investigations in monolayer culture indicated that EMD had a significant dose-dependent stimulatory effect (p < 0.05, n = 6) on the proliferation of bovine primary synovial cells. However, pre-treating culture plates with EMD significantly inhibited cell attachment (p < 0.01, n = 6). EMD's effects on synovial cells, seeded onto ligament scaffolds, were then investigated in several in vitro experiments modelling 3 possible modes for clinical EMD administration (pre-, intra-, and post-operative). In the pre-operative model, EMD was adsorbed onto scaffolds before the addition of cells. In the intra-operative model, EMD and cells were added simultaneously to scaffolds in the culture medium. In the post-operative model, cells were pre-seeded onto scaffolds before EMD was administered. EMD significantly inhibited cell adhesion in the pre-operative model (p < 0.05, n = 6) and had no significant benefit in the intra-operative model. In the post-operative model, the addition of EMD to previously cell-seeded scaffolds significantly increased their total deoxyribonucleic acid content (p < 0.01, n = 5). EMD's stimulative effect on cell proliferation in vitro suggests that it may accelerate scaffold colonization by cells (and in turn tissue induction) in situ. However, its inhibitory effect on synovial cell attachment in vitro implies that it may only be suited to post-operative administration. |
| تدمد: | 1557-8690 1076-3279 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9212598808ff4cb9c39c0422a9b00daa https://doi.org/10.1089/ten.2006.0059 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....9212598808ff4cb9c39c0422a9b00daa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15578690 10763279 |
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