Optimized Production of a Redox Metabolite (pyocyanin) by Pseudomonas aeruginosa NEJ01R Using a Maize By-Product
| العنوان: | Optimized Production of a Redox Metabolite (pyocyanin) by Pseudomonas aeruginosa NEJ01R Using a Maize By-Product |
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| المؤلفون: | Luis Ortiz-Frade, Yolanda Reyes-Vidal, Jesús Alberto Pérez-García, Mayra Leticia Figueroa-Magallón, Francisco Javier Bacame-Valenzuela, Fabricio Espejel-Ayala |
| المصدر: | Microorganisms Volume 8 Issue 10 Microorganisms, Vol 8, Iss 1559, p 1559 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Microbiology (medical), Metabolite, pyocyanin, maize industry wastewater, medicine.disease_cause, 01 natural sciences, Microbiology, Redox, Article, 03 medical and health sciences, chemistry.chemical_compound, redox metabolite, Pyocyanin, electrochemical analysis, 010608 biotechnology, Virology, By-product, medicine, valorization, lcsh:QH301-705.5, response surface analysis, Chromatography, Pseudomonas aeruginosa, Pseudomonas aeruginosa NEJ01R, Substrate (chemistry), Bioproduction, bioproduction, 030104 developmental biology, Membrane, lcsh:Biology (General), chemistry, optimization |
| الوصف: | Pseudomonas aeruginosa metabolizes pyocyanin, a redox molecule related to diverse biological activities. Culture conditions for the production of pyocyanin in a defined medium were optimized using a statistical design and response surface methodology. The obtained conditions were replicated using as substrate an alkaline residual liquid of cooked maize and its by-products. The untreated effluent (raw nejayote, RN) was processed to obtain a fraction without insoluble solids (clarified fraction, CL), then separated by a 30 kDa membrane where two fractions, namely, retentate (RE) and filtered (FI), were obtained. Optimal conditions in the defined medium were 29.6 ° C, 223.7 rpm and pH = 6.92, which produced 2.21 &mu g mL&minus 1 of pyocyanin, and by using the wastewater, it was possible to obtain 3.25 &mu 1 of pyocyanin in the retentate fraction at 40 h. The retentate fraction presented the highest concentration of total solids related to the maximum concentration of pyocyanin (PYO) obtained. The pyocyanin redox behavior was analyzed using electrochemical techniques. In this way, valorization of lime-cooked maize wastewater (nejayote) used as a substrate was demonstrated in the production of a value-added compound, such as pyocyanin, a redox metabolite of Pseudomonas aeruginosa NEJ01R. |
| وصف الملف: | application/pdf |
| تدمد: | 2076-2607 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::928d1a106ec10a1ce636ce209312a9bb https://doi.org/10.3390/microorganisms8101559 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....928d1a106ec10a1ce636ce209312a9bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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