Altered properties and structures of root exudate polysaccharides in a root hairless mutant of barley
| العنوان: | Altered properties and structures of root exudate polysaccharides in a root hairless mutant of barley |
|---|---|
| المؤلفون: | Andrew F Galloway, Jumana Akhtar, Emma Burak, Susan E Marcus, Katie J Field, Ian C Dodd, Paul Knox |
| المصدر: | Plant Physiology. 190:1214-1227 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Epitopes, Soil, Polymers, Polysaccharides, Physiology, Carbohydrates, Genetics, Antibodies, Monoclonal, Hordeum, Exudates and Transudates, Plant Science, Plant Roots |
| الوصف: | Root exudates and rhizosheaths of attached soil are important features of growing roots. To elucidate factors involved in rhizosheath formation, wild-type (WT) barley (Hordeum vulgare L. cv. Pallas) and a root hairless mutant, bald root barley (brb), were investigated with a combination of physiological, biochemical, and immunochemical assays. When grown in soil, WT barley roots bound ∼5-fold more soil than brb per unit root length. High molecular weight (HMW) polysaccharide exudates of brb roots had less soil-binding capacity than those of WT root exudates. Carbohydrate and glycan monoclonal antibody analyses of HMW polysaccharide exudates indicated differing glycan profiles. Relative to WT plants, root exudates of brb had reduced signals for arabinogalactan-protein (AGP), extensin, and heteroxylan epitopes. In contrast, the root exudate of 2-week-old brb plants contained ∼25-fold more detectable xyloglucan epitope relative to WT. Root system immunoprints confirmed the higher levels of release of the xyloglucan epitope from brb root apices and root axes relative to WT. Epitope detection with anion-exchange chromatography indicated that the increased detection of xyloglucan in brb exudates was due to enhanced abundance of a neutral polymer. Conversely, brb root exudates contained decreased amounts of an acidic polymer, with soil-binding properties, containing the xyloglucan epitope and glycoprotein and heteroxylan epitopes relative to WT. We, therefore, propose that, in addition to physically structuring soil particles, root hairs facilitate rhizosheath formation by releasing a soil-binding polysaccharide complex. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::940c13fc83aadaf32e55633e1b0b7f68 https://doi.org/10.1093/plphys/kiac341 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....940c13fc83aadaf32e55633e1b0b7f68 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
|---|