التفاصيل البيبلوغرافية
| العنوان: |
Frequent detection of cell-associated HIV-1 RNA in patients with plasma viral load50 copies/ml |
| المؤلفون: |
Bernd Kupfer, Jürgen K. Rockstroh, Rolf Kaiser, Bertfried Matz, Martin Däumer, Nazifa Qurishi, Ulrich Spengler, Fabienne Roden |
| المصدر: |
Journal of medical virology. 79(10) |
| سنة النشر: |
2007 |
| مصطلحات موضوعية: |
CD4-Positive T-Lymphocytes, Genes, Viral, Molecular Sequence Data, Lipopolysaccharide Receptors, Viremia, HIV Infections, Biology, CD8-Positive T-Lymphocytes, HIV Envelope Protein gp120, Virus, Virology, Blood plasma, medicine, Humans, Amino Acid Sequence, Lymphocytes, RNA, Viral Load, medicine.disease, biology.organism_classification, Peptide Fragments, Infectious Diseases, Cross-Sectional Studies, Viral replication, Viral evolution, Immunology, Lentivirus, HIV-1, RNA, Viral, Viral load, Sequence Alignment |
| الوصف: |
Despite prolonged undetectable plasma viral load some HIV-1 infected patients have been reported to develop resistance-associated mutations leading to treatment failure. The mechanisms for this phenomenon and the point of origin for residual viral evolution are still not elucidated. In order to quantify cell-associated HIV-1 RNA in patients with different levels of plasma viremia paired cell-associated HIV-1 RNA loads and plasma viral loads were determined. Weak inverse correlation between these parameters and the amounts of CD4+ T cells was observed, whereas there was no correlation between viral loads and CD8+ T cells or CD14+ monocytes, respectively. In a subset of patients, cell-associated and plasma HIV-1 env V3 sequences were analyzed. Plasma viral load and the amount of cell-associated HIV-RNA correlated strongly. However, in 62.3% of patients with undetectable plasma viral load cell-associated HIV-RNA could be detected. Analyses of HIV-RNA in plasma and blood cells showed identical sequences in 4/19 patients, whereas the majority of patients had differing HIV-1 RNA sequences in plasma and cells, respectively. In summary, this study shows that residual viral replication in peripheral blood still occurs in the majority of patients with undetectable plasma viral load. Since these replication events could lead to ongoing viral evolution it should be considered to optimize antiretroviral therapy in order to minimize the development of drug resistance. J. Med. Virol. 79:1440–1445, 2007. © Wiley-Liss, Inc. |
| تدمد: |
0146-6615 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::944447065577f98c8820eb466fb456f4
https://pubmed.ncbi.nlm.nih.gov/17705170 |
| حقوق: |
CLOSED |
| رقم الأكسشن: |
edsair.doi.dedup.....944447065577f98c8820eb466fb456f4 |
| قاعدة البيانات: |
OpenAIRE |
