Differentiation Antagonizing Non‐protein Coding RNA Knockdown Alleviates Lipopolysaccharide‐Induced Inflammatory Injury and Apoptosis in Human Chondrocyte Primary Chondrocyte Cells Through Upregulating miRNA‐19a‐3p
| العنوان: | Differentiation Antagonizing Non‐protein Coding RNA Knockdown Alleviates Lipopolysaccharide‐Induced Inflammatory Injury and Apoptosis in Human Chondrocyte Primary Chondrocyte Cells Through Upregulating miRNA‐19a‐3p |
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| المؤلفون: | Xunlu Yin, He Yin, Linghui Li, Hong‐mei Zhang, Xu Wei, Shang‐quan Wang, Ying‐chun Zhao, Gang Sun, Kaiming Li, Xue‐peng Li, Li‐guo Zhu |
| المصدر: | Orthopaedic Surgery Orthopaedic Surgery, Vol 13, Iss 1, Pp 276-284 (2021) |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Lipopolysaccharides, Scientific Articles, Cell, Down-Regulation, Gene Expression, Lipopolysaccharide, Apoptosis, Chondrocyte, 03 medical and health sciences, Chondrocytes, 0302 clinical medicine, lcsh:Orthopedic surgery, Osteoarthritis, microRNA, Gene expression, medicine, Humans, Scientific Article, Orthopedics and Sports Medicine, Viability assay, Inflammatory injury, Inflammation, 030222 orthopedics, Gene knockdown, Chemistry, Cell Differentiation, miR‐19a, Up-Regulation, Cell biology, lcsh:RD701-811, MicroRNAs, RNA silencing, medicine.anatomical_structure, RNA, Long Noncoding, Surgery, Tumor necrosis factor alpha, DANCR, 030217 neurology & neurosurgery |
| الوصف: | Objective To confirm the role of long noncoding RNA differentiation antagonizing non‐protein coding RNA (DANCR) in chondrocyte inflammatory injury in osteoarthritis (OA) in vitro, as well as its molecular mechanism. Methods Human primary chondrocytes were treated with lipopolysaccharide (LPS) to construct a chondrocyte inflammatory injury in human OA cell model. Gene expression was detected using real‐time quantitative polymerase chain reaction. Cell inflammatory injury was evaluated by Cell Counting Kit‐8 assay, flow cytometry, and enzyme‐linked immunosorbent assay. The interplay between miRNA‐19a‐3p (miR‐19a) and DANCR was validated by dual‐luciferase reporter assay and RNA immunoprecipitation. Results Expression of DANCR was upregulated, and miR‐19a was downregulated in human OA cartilage and LPS‐treated primary chondrocytes in vitro. Moreover, DANCR expression was inversely correlated with miR‐19a in OA patients. LPS reduced cell viability and increased the apoptotic rate and secretion of interleukin (IL)‐1β, IL‐6, IL‐8, as well as tumor necrosis factor (TNF)‐α in primary chondrocyte cells in vitro, suggesting an inflammatory injury model of OA. Functionally, knockdown of DANCR could attenuate LPS‐induced apoptosis and inflammatory response, as evidenced by improved cell viability, and reduced apoptotic rate and products of IL‐1β, IL‐6, IL‐8, and TNF‐α. Notably, DANCR negatively regulated miR‐19a expression, presumably via sponging. Furthermore, miR‐19a deletion eliminated the effect of DANCR knockdown on apoptosis and the inflammatory response of primary chondrocytes under LPS stress. Conclusion Differentiation antagonizing non‐protein coding RNA silencing could protect human chondrocyte cells against LPS‐induced inflammatory injury and apoptosis through targeting miR‐19a, suggesting a vital role of the DANCR/miR‐19a axis in OA. Highlights Expression of DANCR and miR‐19a was upregulated and downregulated in human OA cartilage, respectively.Silencing DANCR alleviated LPS‐induced apoptosis and inflammatory response in primary chondrocytes, which could be counteracted by deleting miR‐19a.DANCR functioned as a sponge for miR‐19a. |
| تدمد: | 1757-7861 1757-7853 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9455bb0cc960dbcecb4fde982fba0f5c https://doi.org/10.1111/os.12845 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9455bb0cc960dbcecb4fde982fba0f5c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17577861 17577853 |
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