Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models
| العنوان: | Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models |
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| المؤلفون: | Zhongping Chen, Hai-Ping Cai, Zhi-Hui Yu, Yi-Ying Zhao, Guokai Feng, Xiang-Rong Ni, Fu-Rong Chen, Jing Wang, Yan-Jiao Yu |
| المصدر: | Journal of Neuro-Oncology. 148:245-258 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cancer Research, Near-Infrared Fluorescence Imaging, genetic structures, Transferrin receptor, urologic and male genital diseases, Blood–brain barrier, Mice, 03 medical and health sciences, 0302 clinical medicine, Live cell imaging, Cell Line, Tumor, Glioma, Receptors, Transferrin, medicine, Animals, Fluorescent Dyes, Brain Neoplasms, urogenital system, business.industry, Optical Imaging, technology, industry, and agriculture, Carbocyanines, medicine.disease, Xenograft Model Antitumor Assays, nervous system diseases, Disease Models, Animal, medicine.anatomical_structure, Microscopy, Fluorescence, Neurology, Oncology, 030220 oncology & carcinogenesis, Cancer research, Fluorescein, Neurology (clinical), Molecular imaging, business, 030217 neurology & neurosurgery, Homing (hematopoietic), Glioblastoma |
| الوصف: | Optical molecular imaging technology that indiscriminately detects intracranial glioblastoma (GBM) can help neurosurgeons effectively remove tumor masses. Transferrin receptor 1 (TfR 1) is a diagnostic and therapeutic target in GBM. A TfR 1-targeted peptide, CRTIGPSVC (CRT), was shown to cross the blood brain barrier (BBB) and accumulate at high levels in GBM tissues. In this study, we synthesized a TfR 1-targeted near-infrared fluorescent (NIRF) probe, Cy5-CRT, for identifying the GBM tissue margin in mouse models. We initially confirmed the overexpression of TfR 1 in GBM and the tumor-specific homing ability of Cy5-CRT in subcutaneous and orthotopic GBM mouse models. We then examined the feasibility of Cy5-CRT for identifying the tumor margin in orthotopic GBM xenografts. Finally, we compared Cy5-CRT with the clinically used fluorescein sodium in identifying tumor margins. Cy5-CRT specifically accumulated in GBM tissues and detected the tumor burden with exceptional contrast in mice with orthotopic GBM, enabling fluorescence-guided GBM resection under NIRF live imaging conditions. Importantly, Cy5-CRT recognized the GBM tissue margin more clearly than fluorescein sodium. The TfR 1-targeted optical probe Cy5-CRT specifically differentiates tumor tissues from the surrounding normal brain with high sensitivity, indicating its potential application for the precise surgical removal of GBM. |
| تدمد: | 1573-7373 0167-594X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94ad405c8f2e6f6e9af5dd41671ec275 https://doi.org/10.1007/s11060-020-03527-3 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....94ad405c8f2e6f6e9af5dd41671ec275 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15737373 0167594X |
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