Urine Stasis Predisposes to Urinary Tract Infection by an Opportunistic Uropathogen in the Megabladder (Mgb) Mouse
العنوان: | Urine Stasis Predisposes to Urinary Tract Infection by an Opportunistic Uropathogen in the Megabladder (Mgb) Mouse |
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المؤلفون: | Birong Li, Brian Becknell, Susan E. Ingraham, Michael E. Wilhide, Ahmad Z. Mohamed |
المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 9, p e0139077 (2015) |
بيانات النشر: | Public Library of Science (PLoS), 2015. |
سنة النشر: | 2015 |
مصطلحات موضوعية: | medicine.medical_specialty, Bacteriuria, Struvite, Staphylococcus, medicine.medical_treatment, Urinary system, Urinary Bladder, 030232 urology & nephrology, Urology, lcsh:Medicine, Magnesium Compounds, Urine, Urinary Diversion, urologic and male genital diseases, Phosphates, Mice, 03 medical and health sciences, 0302 clinical medicine, Urolithiasis, Cystitis, Animals, Medicine, Urothelium, Risk factor, lcsh:Science, Urinary Tract, 030304 developmental biology, 0303 health sciences, Multidisciplinary, Urinary bladder, Pyelonephritis, business.industry, lcsh:R, Urinary diversion, Aplasia, Hydrogen-Ion Concentration, bacterial infections and mycoses, medicine.disease, Mice, Mutant Strains, female genital diseases and pregnancy complications, 3. Good health, medicine.anatomical_structure, Urinary Tract Infections, lcsh:Q, Female, Disease Susceptibility, business, Research Article |
الوصف: | Purpose Urinary stasis is a risk factor for recurrent urinary tract infection (UTI). Homozygous mutant Megabladder (Mgb-/-) mice exhibit incomplete bladder emptying as a consequence of congenital detrusor aplasia. We hypothesize that this predisposes Mgb-/- mice to spontaneous and experimental UTI. Methods Mgb-/-, Mgb+/-, and wild-type female mice underwent serial ultrasound and urine cultures at 4, 6, and 8 weeks to detect spontaneous UTI. Urine bacterial isolates were analyzed by Gram stain and speciated. Bladder stones were analyzed by x-ray diffractometry. Bladders and kidneys were subject to histologic analysis. The pathogenicity of coagulase-negative Staphylococcus (CONS) isolated from Mgb-/- urine was tested by transurethral administration to culture-negative Mgb-/- or wild-type animals. The contribution of urinary stasis to CONS susceptibility was evaluated by cutaneous vesicostomy in Mgb-/- mice. Results Mgb-/- mice develop spontaneous bacteriuria (42%) and struvite bladder stones (31%) by 8 weeks, findings absent in Mgb+/- and wild-type controls. CONS was cultured as a solitary isolate from Mgb-/- bladder stones. Bladders and kidneys from mice with struvite stones exhibit mucosal injury, inflammation, and fibrosis. These pathologic features of cystitis and pyelonephritis are replicated by transurethral inoculation of CONS in culture-negative Mgb-/- females, whereas wild-type animals are less susceptible to CONS colonization and organ injury. Cutaneous vesicostomy prior to CONS inoculation significantly reduces the quantity of CONS recovered from Mgb-/- urine, bladders, and kidneys. Conclusions CONS is an opportunistic uropathogen in the setting of urinary stasis, leading to enhanced UTI incidence and severity in Mgb-/- mice. |
تدمد: | 1932-6203 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::957456b0a79c3ed4095289cd4608ac1d https://doi.org/10.1371/journal.pone.0139077 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....957456b0a79c3ed4095289cd4608ac1d |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19326203 |
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