Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens
| العنوان: | Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens |
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| المؤلفون: | Fatemeh Iranpour, Peyman Faridifar, Seyed Fazlollah Mousavi, Fakhri Haghi, Bahman Mirzaei, Mostafa Shahmoradi, Reza Shapouri, Reyhaneh Babaei, Mahnaz Ezzedin |
| المصدر: | BMC Research Notes, Vol 13, Iss 1, Pp 1-6 (2020) BMC Research Notes |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Prosthesis-Related Infections, Genotype, medicine.drug_class, icaADCB, 030106 microbiology, Antibiotics, lcsh:Medicine, Microbial Sensitivity Tests, Multiplex colony PCR, Biology, Polymerase Chain Reaction, General Biochemistry, Genetics and Molecular Biology, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Staphylococcus epidermidis, Clavulanic acid, Drug Resistance, Bacterial, medicine, Humans, 030212 general & internal medicine, Cefamandole, lcsh:Science (General), lcsh:QH301-705.5, lcsh:R, Biofilm, sdrG, General Medicine, atlE, Staphylococcal Infections, Amoxicillin, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Biofilm-related genes, Anti-Bacterial Agents, Housekeeping gene, Research Note, Phenotype, lcsh:Biology (General), Amikacin, Biofilms, medicine.drug, lcsh:Q1-390 |
| الوصف: | Objectives Staphylococcus epidermidis is the primary causative agent of infections associated with indwelling biomaterials. Antibiotic susceptibility patterns, Biofilm formation capability, and screening of responsible genes in biofilm formation procedure in clinical isolates (icaA, icaB, icaC, icaD, sdrG, and atlE) were assigned as the main objectives in this study. The clinical samples were analyzed via standard biochemical assays for identifying different bacteria which were confirmed using the multiplex colony PCR method. Subsequently, biofilm-formation capability, antibiotic susceptibility testing, and the frequency of genes responsible for biofilm formation in the confirmed strains were checked. Results Out of 183 clinical specimens 54 S. epidermidis isolates were detected by targeting a housekeeping gene (sesc) taking advantage of the PCR procedure. All of the strains were Biofilm forming producers. The in vitro biofilm formation assays determined that 45 (83.33%), 5 (9.26%), 4 (7.41%) were strong, moderate, and weak biofilm former strains respectively. Among the isolated strains, the specific frequencies of the biofilm-forming genes were specified to be (98%) for sdrG, (84%) for atlE, (80%) for icaC, and (70%) for icaD. Cefamandole and Amikacin are the most effective antibiotics in isolated strains. All strains were ascertained to be methicillin and amoxicillin/clavulanic acid resistant. |
| اللغة: | English |
| تدمد: | 1756-0500 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95a0489cd37ee07c47e86c7573f89503 http://link.springer.com/article/10.1186/s13104-020-04965-y |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....95a0489cd37ee07c47e86c7573f89503 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17560500 |
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