Microwave ablation ofex vivohuman liver and colorectal liver metastases with a novel 14.5 GHz generator
| العنوان: | Microwave ablation ofex vivohuman liver and colorectal liver metastases with a novel 14.5 GHz generator |
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| المؤلفون: | Hassan Malik, Paula Ghaneh, Stephen W. Fenwick, M. Terlizzo, Christopher Paul Hancock, G.J. Poston, Neil R. Kitteringham, D. Dunne, Robert P. Jones |
| المصدر: | International Journal of Hyperthermia. 28:43-54 |
| بيانات النشر: | Informa UK Limited, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Ablation Techniques, Male, Cancer Research, Pathology, medicine.medical_specialty, Physiology, medicine.medical_treatment, Cell, Physiology (medical), medicine, Humans, Microwaves, Aged, Human liver, business.industry, Liver Neoplasms, Microwave ablation, Cancer, Middle Aged, medicine.disease, Ablation, medicine.anatomical_structure, Liver, Female, Colorectal Neoplasms, business, Ex vivo, Ablation zone |
| الوصف: | This study assessed the relationship between time, power and ablation size using a novel high-frequency 14.5 GHz microwave applicator in ex vivo human hepatic parenchyma and colorectal liver metastases. Previous examination has demonstrated structurally normal but non-viable cells within the ablation zone. This study aimed to further investigate how ablation affects these cells, and to confirm non-viability.Ablations were performed in ex vivo human hepatic parenchyma and tumour for a variety of time (10-180 s) and power (10-50 W) settings. Histological examination was performed to assess cellular anatomy, whilst enzyme histochemistry was used to confirm cellular non-viability. Transmission electron microscopy was used to investigate the subcellular structural effects of ablation within these fixed cells. Preliminary proteomic analysis was also performed to explore the mechanism of microwave cell death.Increasing time and power settings led to a predictable and reproducible increase in size of ablation. At 50 W and 180 s application, a maximum ablation diameter of 38.8 mm (±1.3) was produced. Ablations were produced rapidly, and at all time and power settings ablations remained spherical (longest:shortest diameter1.2). Routine histological analysis using haematoxylin-eosin (HE) confirmed well preserved cellular anatomy despite ablation. Transmission electron microscopy demonstrated marked subcellular damage. Enzyme histochemistry showed complete absence of viability in ablated tissue.Large spherical ablation zones can be rapidly and reproducibly achieved in ex vivo human hepatic parenchyma and colorectal liver metastases using a 14.5 GHz microwave generator. Despite well preserved cellular appearance, ablated tissue is non-viable. |
| تدمد: | 1464-5157 0265-6736 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95c6f4e2bcf4b9c30641a51c503dce83 https://doi.org/10.3109/02656736.2011.610428 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....95c6f4e2bcf4b9c30641a51c503dce83 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 14645157 02656736 |
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