Human Placental Vascular Development: Vasculogenic and Angiogenic (Branching and Nonbranching) Transformation Is Regulated by Vascular Endothelial Growth Factor-A, Angiopoietin-1, and Angiopoietin-2
| العنوان: | Human Placental Vascular Development: Vasculogenic and Angiogenic (Branching and Nonbranching) Transformation Is Regulated by Vascular Endothelial Growth Factor-A, Angiopoietin-1, and Angiopoietin-2 |
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| المؤلفون: | Eli Geva, Charles Zaloudek, Robert B. Jaffe, Annette T. Byrne, David G. Ginzinger, Dan H. Moore |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 87:4213-4224 |
| بيانات النشر: | The Endocrine Society, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, medicine.medical_specialty, Transcription, Genetic, Angiogenesis, Placenta, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Neovascularization, Physiologic, Blood Pressure, Endothelial Growth Factors, Biology, Polymerase Chain Reaction, Biochemistry, Angiopoietin-2, Angiopoietin, chemistry.chemical_compound, Endocrinology, Vasculogenesis, Syncytiotrophoblast, Pregnancy, Reference Values, Internal medicine, Angiopoietin-1, medicine, Humans, RNA, Messenger, DNA Primers, Membrane Glycoproteins, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Macrophages, Biochemistry (medical), Gene Expression Regulation, Developmental, Proteins, Vascular endothelial growth factor, Vascular endothelial growth factor A, medicine.anatomical_structure, chemistry, Chorionic villi, Female |
| الوصف: | During placental development, vessel formation occurs initially by vasculogenesis and subsequently by branching and nonbranching angiogenesis. We investigated vascular endothelial growth factor (VEGF)-A, angiopoietin (Ang)-1 and -2 transcript profiles, and the protein products that they encode in placentas from normotensive pregnancies throughout pregnancy. In addition, we compared these genes in placentas from normotensive women and those with preeclampsia during the third trimester. Quantitative real-time PCR analysis demonstrated that VEGF-A and Ang1 mRNA increased in a linear pattern by 2.5 (not significant) and 2.8%/wk (P = 0.034), respectively, whereas Ang2 decreased logarithmically by 3.5%/wk (P = 0.0003). Ang2 mRNA was 400- and 100-fold higher than Ang1 and VEGF-A, respectively, in the first trimester and declined to 20-fold and 7-fold in the third. Ang2 protein (ELISA) decreased by 4.7%/wk (P = 0.0001), whereas Ang1 and VEGF-A were undetectable. In preeclampsia compared with normotensive pregnancy, only VEGF-A mRNA increased significantly, by 3-fold (P = 0.006). This increase may be related to low oxygen tension, as VEGF-A is up-regulated by hypoxia. In situ hybridization and immunohistochemical studies revealed that VEGF-A was localized in cyto- and syncytiotrophoblast and perivascular cells, whereas Ang1 and Ang2 were only in syncytiotrophoblast and perivascular cells in the immature intermediate villi during the first and second trimesters, and mature intermediate and terminal villi during the third trimester. These data suggest that these molecules may play important roles in placental biology and chorionic villus vascular development and remodeling in an autocrine/paracrine manner. The tight correlation between Ang2 mRNA and protein indicates that regulation of placental vascular development occurs at the transcriptional, and not translational, level. |
| تدمد: | 1945-7197 0021-972X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95e4807ebe948c6d5e0559d0f871914d https://doi.org/10.1210/jc.2002-020195 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....95e4807ebe948c6d5e0559d0f871914d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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