Transgenic Analysis of the Stem Cell Leukemia +19 Stem Cell Enhancer in Adult and Embryonic Hematopoietic and Endothelial Cells
| العنوان: | Transgenic Analysis of the Stem Cell Leukemia +19 Stem Cell Enhancer in Adult and Embryonic Hematopoietic and Endothelial Cells |
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| المؤلفون: | María José Sánchez, Berthold Göttgens, Lev Silberstein, Sandie Piltz, Anthony R. Green, Merav Socolovsky, Mark Bowen, Gary J. Hoffman, Sarah Kinston, Laure Gambardella, Ying Liu |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Oxford University Press (OUP), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Male, Cellular differentiation, Bone Marrow Cells, Mice, Transgenic, Biology, Hematopoietic stem cell, Gene Expression Regulation, Enzymologic, Mice, Transcriptional regulation, Proto-Oncogene Proteins, Basic Helix-Loop-Helix Transcription Factors, medicine, Animals, Cell Lineage, Mast Cells, Enhancer, T-Cell Acute Lymphocytic Leukemia Protein 1, Induced stem cells, Endothelial Cells, Gene Expression Regulation, Developmental, Cell Biology, Hematopoietic Stem Cells, beta-Galactosidase, Embryonic stem cell, Cell biology, Mice, Inbred C57BL, Endothelial stem cell, Enhancer Elements, Genetic, medicine.anatomical_structure, Mice, Inbred CBA, Molecular Medicine, Female, Stem cell, Transcription factor SCL/tal-1, Megakaryocytes, Experimental models, Developmental Biology, Adult stem cell |
| الوصف: | 11 páginas, 5 figuras, 1 tabla. Appropriate transcriptional regulation is critical for the biological functions of many key regulatory genes, including the stem cell leukemia (SCL) gene. As part of a systematic dissection of SCL transcriptional regulation, we have previously identified a 5,245-bp SCL +18/19 enhancer that targeted embryonic endothelium together with embryonic and adult hematopoietic progenitors and stem cells (HSCs). This enhancer is proving to be a powerful tool for manipulating hematopoietic progenitors and stem cells, but the design and interpretation of such transgenic studies require a detailed understanding of enhancer activity in vivo. In this study, we demonstrate that the +18/19 enhancer is active in mast cells, megakaryocytes, and adult endothelium. A 644-bp +19 core enhancer exhibited similar temporal and spatial activity to the 5,245-bp +18/19 fragment both during development and in adult mice. Unlike the +18/19 enhancer, the +19 core enhancer was only active in adult mice when linked to the eukaryotic reporter gene human placental alkaline phosphatase. Activity of a single core enhancer in HSCs, endothelium, mast cells, and megakaryocytes suggests possible overlaps in their respective transcriptional programs. Moreover, activity in a proportion of thymocytes and other SCL-negative cell types suggests the existence of a silencer elsewhere in the SCL locus. Work in the authors' laboratory is funded by the Leukaemia Research Fund, Wellcome Trust, BBSRC, and Cambridge MIT Institute. M.S. was supported by a National Cancer Institute Howard Temin (KO1) Award. M.J.S. was supported through an MRC career development award. |
| تدمد: | 1549-4918 1066-5099 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95fcf118a5395c4d1cc0371c99d579c8 https://doi.org/10.1634/stemcells.2005-0090 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....95fcf118a5395c4d1cc0371c99d579c8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15494918 10665099 |
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