Association of Asef and Cdc42 Expression to Tubular Injury in Diseased Human Kidney
| العنوان: | Association of Asef and Cdc42 Expression to Tubular Injury in Diseased Human Kidney |
|---|---|
| المؤلفون: | Hui-Teng Cheng, Cheng Han Chao, Liang-Chin Chen, Lih-Yuan Lin, I Pu Juang |
| المصدر: | Journal of Investigative Medicine. 61:1097-1103 |
| بيانات النشر: | SAGE Publications, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Adult, Male, Pathology, medicine.medical_specialty, Adolescent, Cell division, Cellular polarity, CDC42, Biology, General Biochemistry, Genetics and Molecular Biology, Young Adult, Biopsy, medicine, Humans, cdc42 GTP-Binding Protein, Aged, 80 and over, Kidney, medicine.diagnostic_test, Tight junction, General Medicine, Middle Aged, Cell cycle, Kidney Tubules, medicine.anatomical_structure, Gene Expression Regulation, Female, Kidney Diseases, Guanine nucleotide exchange factor, Rho Guanine Nucleotide Exchange Factors, Glomerular Filtration Rate |
| الوصف: | Background Cdc42 is a small guanosine-5′-triphosphatase of the Rho family and plays essential roles in the establishment of cellular polarity and tight junctions in epithelial cells. Adenomatous polyposis coli-associated exchange factor (Asef) is a canonical guanine nucleotide exchange factor of Cdc42 and renders Cdc42 to be guanosine-5′-triphosphate bound and activated. The expression patterns and their significance in human renal diseases are unknown. Methods We examined the expression of Cdc42 and Asef in kidney biopsy specimens of 15 patients and in normal kidney tissue using immunofluorescence and correlated the expression patterns with the clinical characteristics. We also analyzed the coexpression pattern of Ki-67, a marker indicating cell division, and Asef in selected patients. Results Expression of Asef and Cdc42 together was associated with tubular injury with 100% specificity. Expression of Asef, regardless of Cdc42, also showed a significant diagnostic odds ratio for the presence of the injury. Expression of Asef was associated with lower estimated glomerular filtration rate at the time of biopsy and larger area of interstitial fibrosis. All Ki-67–expressing tubular cells expressed Asef. Conclusions Induction of Asef and Cdc42 in the renal tubules is a cellular response to injury. Asef induction seems a necessary step for injured tubular cells to enter cell cycle. |
| تدمد: | 1708-8267 1081-5589 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96243a39ca8cb3d8542116f0dea6dec7 https://doi.org/10.2310/jim.0b013e3182a32fdb |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....96243a39ca8cb3d8542116f0dea6dec7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17088267 10815589 |
|---|