NMR profiling of transgenic peas
| العنوان: | NMR profiling of transgenic peas |
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| المؤلفون: | Adrian J. Charlton, Phil Mullineaux, Stephen Holmes, Sarah Oehlschlager, Samantha Bean, James Chisholm, Theo R. Allnutt, Noel Ellis |
| المصدر: | Plant Biotechnology Journal. 2:27-35 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Genetics, biology, Metabolite, Transgene, food and beverages, Plant Science, Nuclear magnetic resonance spectroscopy, biology.organism_classification, Pisum, Genetically modified organism, chemistry.chemical_compound, Sativum, chemistry, Botany, Proton NMR, Transgenic lines, Agronomy and Crop Science, Biotechnology |
| الوصف: | A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. Multivariate analysis of 1H NMR (nuclear magnetic resonance) spectra obtained from uniformly grown glasshouse plants revealed differences between the transgenic and control group that exceeded the natural variation of the plants. When a larger data set of six related transgenic lines was analysed, including a null segregant in addition to the wild-type control, multivariate analysis showed that the distribution of metabolites in the transgenics was different from that of the null segregant. However, the profile obtained from the wild-type material was diverse in comparison with both the transgenics and the null segregant, suggesting that the primary cause of the observed differences was that the transformation process selects for a subset of individuals able to undergo the transformation and selection procedures, and that their descendants have a restricted variation in metabolite profile, rather than that the presence of the transgene itself generates these differences. |
| تدمد: | 1467-7652 1467-7644 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9730aa65e04a10c61cd0d7401d641d1b https://doi.org/10.1046/j.1467-7652.2003.00045.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9730aa65e04a10c61cd0d7401d641d1b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14677652 14677644 |
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