RNAi-Mediated Suppression of Laccase2 Impairs Cuticle Tanning and Molting in the Cotton Boll Weevil (Anthonomus grandis)
| العنوان: | RNAi-Mediated Suppression of Laccase2 Impairs Cuticle Tanning and Molting in the Cotton Boll Weevil (Anthonomus grandis) |
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| المؤلفون: | Isabela Tristan Lourenço-Tessutti, Diogo Martins-de-Sa, Marilia Santos Silva, Janice de Almeida Engler, Walter R. Terra, Daniele H. Pinheiro, Fabrício Barbosa Monteiro Arraes, Roberta Ramos Coelho, José Dijair Antonino, Maria Cristina Mattar da Silva, Maria Fatima Grossi-de-Sa, Alexandre Augusto Pereira Firmino, Clidia Eduarda Moreira-Pinto, Fernando Campos de Assis Fonseca, Leonardo Lima Pepino de Macedo |
| المساهمون: | Embrapa Recursos Genéticos e Biotecnologia [Brasília], Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, University of Brasilia [Brazil] (UnB), Universidade Federal Rural de Pernambuco (UFRPE), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), National Institute of Science and Technology (INCT), Brazilian Agricultural Research Corporation (Embrapa), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Département Santé des Plantes et Environnement (DPT SPE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of São Paulo (USP), Universidade Católica de Brasília (UCB), Associação Brasileira dos Produtores de Algodão, INCT – Plant Stress Biotech (Grant Numbers 193.001.265/2017 and 465480/2014-4, ALEXANDRE AUGUSTO PEREIRA FIRMINO, DANIELE HELOÍSA PINHEIRO, CLIDIA EDUARDA MOREIRA-PINTO, UNB, JOSÉ DIJAIR ANTONINO, UFRPE, LEONARDO LIMA PEPINO DE MACEDO, Cenargen, DIOGO MARTINS-DE-SA, UNB, FABRÍCIO BARBOSA MONTEIRO ARRAES, UFRGS, ROBERTA RAMOS COELHO, FERNANDO CAMPOS DE ASSIS FONSECA, UNB, MARIA CRISTINA MATTAR DA SILVA, Cenargen, JANICE DE ALMEIDA ENGLER, INCT PLANT STRESS BIOTECH, MARILIA SANTOS SILVA, Cenargen, ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen, WALTER RIBEIRO TERRA, USP, MARIA FATIMA GROSSI DE SA, Cenargen. |
| المصدر: | Frontiers in Physiology Frontiers in Physiology, Frontiers, 2020, 11, pp.591569. ⟨10.3389/fphys.2020.591569⟩ Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Frontiers in Physiology, Vol 11 (2020) Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| بيانات النشر: | Frontiers Media SA, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Integrated pest management, Physiology, media_common.quotation_subject, Cuticle, Insect pest control, Laccase2, Insect, Biology, 01 natural sciences, lcsh:Physiology, gene silencing, 03 medical and health sciences, RNA interference, Physiology (medical), Botany, media_common, 2. Zero hunger, Larva, Cuticle tanning, lcsh:QP1-981, [SDV.BA]Life Sciences [q-bio]/Animal biology, fungi, Gene silencing, biology.organism_classification, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Pupa, cuticle tanning, 010602 entomology, 030104 developmental biology, CONTROLE DE PRAGAS, insect pest control, Anthonomus, RNAi, PEST analysis |
| الوصف: | International audience; The cotton boll weevil, Anthonomus grandis, is the most economically important pest of cotton in Brazil. Pest management programs focused on A. grandis are based mostly on the use of chemical insecticides, which may cause serious ecological impacts. Furthermore, A. grandis has developed resistance to some insecticides after their long-term use. Therefore, alternative control approaches that are more sustainable and have reduced environmental impacts are highly desirable to protect cotton crops from this destructive pest. RNA interference (RNAi) is a valuable reverse genetics tool for the investigation of gene function and has been explored for the development of strategies to control agricultural insect pests. This study aimed to evaluate the biological role of the Laccase2 (AgraLac2) gene in A. grandis and its potential as an RNAi target for the control of this insect pest. We found that AgraLac2 is expressed throughout the development of A. grandis with significantly higher expression in pupal and adult developmental stages. In addition, the immunolocalization of the AgraLac2 protein in third-instar larvae using specific antibodies revealed that AgraLac2 is distributed throughout the epithelial tissue, the cuticle and the tracheal system. We also verified that the knockdown of AgraLac2 in A. grandis resulted in an altered cuticle tanning process, molting defects and arrested development. Remarkably, insects injected with dsAgraLac2 exhibited defects in cuticle hardening and pigmentation. As a consequence, the development of dsAgraLac2-treated insects was compromised, and in cases of severe phenotypic defects, the insects subsequently died. On the contrary, insects subjected to control treatments did not show any visible phenotypic defects in cuticle formation and successfully molted to the pupal and adult stages. Taken together, our data indicate that AgraLac2 is involved in the cuticle tanning process in A. grandis and may be a promising target for the development of RNAi-based technologies. |
| تدمد: | 1664-042X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::975da0e19fde0801d058a473ab60b90a https://doi.org/10.3389/fphys.2020.591569 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....975da0e19fde0801d058a473ab60b90a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664042X |
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