Charged residues on the side of the nucleosome contribute to normal Spt16-gene interactions in budding yeast
| العنوان: | Charged residues on the side of the nucleosome contribute to normal Spt16-gene interactions in budding yeast |
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| المؤلفون: | Michelle L Huynh, Eugene Nyamugenda, Ryan C Banning, Claire E. Turkal, Sarah Marshall, Jacob B. Pierce, Catey May, Andrea A. Duina, A. Brandon Cox |
| المصدر: | Epigenetics. 13:1-7 |
| بيانات النشر: | Informa UK Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Chromatin Immunoprecipitation, Cancer Research, Saccharomyces cerevisiae Proteins, Mutant, Saccharomyces cerevisiae, medicine.disease_cause, Histones, 03 medical and health sciences, 0302 clinical medicine, Gene Expression Regulation, Fungal, medicine, Nucleosome, Molecular Biology, Gene, Genetics, Mutation, Alanine, biology, Brief Report, FACT complex, biology.organism_classification, Nucleosomes, Chromatin, Proton-Translocating ATPases, 030104 developmental biology, Histone, Amino Acid Substitution, biology.protein, Transcriptional Elongation Factors, 030217 neurology & neurosurgery |
| الوصف: | Previous work in Saccharomyces cerevisiae identified three residues located in close proximity to each other on the side of the nucleosome whose integrity is required for proper association of the Spt16 component of the FACT complex across transcribed genes. In an effort to gain further insights into the parameters that control Spt16 interactions with genes in vivo, we tested the effects of additional histone mutants on Spt16 occupancy across two constitutively transcribed genes. These studies revealed that mutations in several charged residues in the vicinity of the three residues originally identified as important for Spt16-gene interactions also significantly perturb normal association of Spt16 across genes. Based on these and our previous findings, we propose that the charge landscape across the region encompassed by these residues, which we refer to as the Influences Spt16-Gene Interactions or ISGI region, is an important contributor to proper Spt16-gene interactions in vivo. |
| تدمد: | 1559-2308 1559-2294 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::979bdcfff2a25b5fd968db458ef919c5 https://doi.org/10.1080/15592294.2017.1418132 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....979bdcfff2a25b5fd968db458ef919c5 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15592308 15592294 |
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