Inorganic nanoparticles as potential regulators of immune response in dendritic cells
| العنوان: | Inorganic nanoparticles as potential regulators of immune response in dendritic cells |
|---|---|
| المؤلفون: | Angela Silvano, Silvia Fogli, Costanza Montis, Debora Berti, Elena Michelucci, Alberto Bosi, Sara Paccosi, Astrid Parenti, Paolo Romagnoli |
| المصدر: | Nanomedicine. 12:1647-1660 |
| بيانات النشر: | Future Medicine Ltd, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | inorganic chemicals, medicine.medical_treatment, education, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, Protein Corona, 02 engineering and technology, Lymphocyte proliferation, Development, Biology, 010402 general chemistry, 01 natural sciences, Immune system, Coated Materials, Biocompatible, Cancer immunotherapy, Antigen, Cell Line, Tumor, Neoplasms, cancer immunotherapy, dendritic cells, lysate, nanoparticles, protein corona, medicine, Humans, General Materials Science, Cytotoxicity, health care economics and organizations, technology, industry, and agriculture, Dendritic Cells, Immunotherapy, respiratory system, Silicon Dioxide, 021001 nanoscience & nanotechnology, Molecular biology, 0104 chemical sciences, Cell biology, Cancer cell, Nanoparticles, Adsorption, Gold, 0210 nano-technology |
| الوصف: | Aim: The spontaneous adsorption of proteins on nanoparticles (NPs) in biological media is exploited to prepare complexes of NPs and proteins from cancer cells’ lysates for application in cancer immunotherapy. Materials & methods: Gold (Au) and silica NPs were synthesized, incubated with cancer cells’ lysates and characterized. Dendritic cells (DCs) were challenged with protein-coated NPs, their maturation, viability and morphology were evaluated and lymphocytes T proliferation was determined. Results: Silica and Au NPs bound different pools of biomolecules from lysates, and are therefore promising selective carriers for antigens. When incubated with immature DCs, NPs were efficiently endocytosed without cytotoxicity. Finally, protein-coated AuNPs promoted DC maturation and DC-mediated lymphocyte proliferation, at variance with lysate alone and protein-coated silica NPs, that did not promote DCs maturation. Conclusion: These results demonstrate that the spontaneous formation of protein coronas on NPs represents a possible approach to fast, easy, cost-effective DCs stimulation. |
| تدمد: | 1748-6963 1743-5889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97ba9cd211a8c9fbb5027c8f86a9a885 https://doi.org/10.2217/nnm-2017-0061 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....97ba9cd211a8c9fbb5027c8f86a9a885 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 17486963 17435889 |
|---|