Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus
| العنوان: | Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus |
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| المؤلفون: | Padma Srikanth, Preethi Radhakrishnan, Rubini Anbalagan, Krishna G Seshadri, Monika Mani, Ramya Barani |
| المصدر: | Indian Journal of Medical Microbiology, Vol 37, Iss 1, Pp 54-59 (2019) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Saliva, polymerase chain reaction, 030106 microbiology, Immunology, lcsh:QR1-502, Microbiology, lcsh:Microbiology, Periodontal pathogen, law.invention, 03 medical and health sciences, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), law, Diabetes mellitus, Glycaemic control, medicine, Immunology and Allergy, 030212 general & internal medicine, Porphyromonas gingivalis, Polymerase chain reaction, Periodontitis, Doxycycline, saliva, General Immunology and Microbiology, biology, business.industry, Type 2 Diabetes Mellitus, medicine.disease, biology.organism_classification, Infectious Diseases, business, medicine.drug |
| الوصف: | Background: Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. Aim and Objectives: A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples. Materials and Methods: DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples. Results:P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914). Conclusion: Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status. |
| اللغة: | English |
| تدمد: | 1998-3646 0255-0857 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97dc59349ae7f2746cf13adb14c3dfef http://www.ijmm.org/article.asp?issn=0255-0857;year=2019;volume=37;issue=1;spage=54;epage=59;aulast=Radhakrishnan |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....97dc59349ae7f2746cf13adb14c3dfef |
| قاعدة البيانات: | OpenAIRE |
Full Text via ClinicalKey | تدمد: | 19983646 02550857 |
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