Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis
| العنوان: | Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis |
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| المؤلفون: | Anthony D. Ho, Linda Viol, Patrick Wuchter, Bernd Giebel, Florian Murke, Gislene Pereira, Annett Neuner, Shoji Hata, Ana Pastor-Peidro |
| المصدر: | The Journal of Cell Biology |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | musculoskeletal diseases, Time Factors, Centriole, Medizin, Mitosis, Retinal Pigment Epithelium, Biology, Article, Cell Line, Mice, 03 medical and health sciences, Mammary Glands, Animal, 0302 clinical medicine, Ciliogenesis, Animals, Humans, NIMA-Related Kinases, Cilia, Kinase activity, Centrioles, 030304 developmental biology, 0303 health sciences, Binding Sites, Cilium, Epithelial Cells, Cell Biology, Cell cycle, Hematopoietic Stem Cells, Cell biology, G2 Phase Cell Cycle Checkpoints, Centrosome, Microtubule Proteins, Female, Interphase, 030217 neurology & neurosurgery, Protein Binding, Signal Transduction, Cell Cycle and Division |
| الوصف: | Viol et al. show that the conserved Never-in-mitosis-A–related kinase-2 (Nek2) removes distal appendage components from the mother centriole prior to mitosis in every cell cycle. These findings suggest that Nek2 prevents cilia maintenance during mitosis via distal appendage regulation. Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs lose their centrosomal localization at the G2/M transition in a manner that relies upon Nek2 kinase activity to ensure low DA levels at mitotic centrosomes. Overexpression of active Nek2A, but not kinase-dead Nek2A, prematurely displaced DAs from the interphase centrosomes of immortalized retina pigment epithelial (RPE1) cells. This dramatic impact was also observed in mammary epithelial cells with constitutively high levels of Nek2. Conversely, Nek2 knockout led to incomplete dissociation of DAs and cilia in mitosis. As a consequence, we observed the presence of a cilia remnant that promoted the asymmetric inheritance of ciliary signaling components and supported cilium reassembly after cell division. Together, our data establish Nek2 as an important kinase that regulates DAs before mitosis. Graphical Abstract |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::984f9f164d1ab3fbd79b1baefd9972fa https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&origin=inward&scp=85082380212 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....984f9f164d1ab3fbd79b1baefd9972fa |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |