FOXO3 induces autophagic flux. a SH-EP/FOXO3-shCtr and SH-EP/FOXO3-shDEPP-13 cells were treated with 50 nM 4OHT and 100 μM CQ for 8 h. LC3-I/LC3-II and p62 expression were assessed by immunoblot analyses. GAPDH served as loading control. Densitometric analyses were performed with the ImageJ 1.48 software. Control (Ctr.) and CQ-treated cells were set as 100%. Shown are mean values ± s.e.m of three independent experiments; statistical analysis was done with the Student’s unpaired t-test, *P
