Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant
| العنوان: | Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant |
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| المؤلفون: | Keiko Sugaya, Yoshie Ishihara, Sonoe Inoue, Kimihiko Sugaya |
| المصدر: | American Journal of Molecular Biology. :38-44 |
| بيانات النشر: | Scientific Research Publishing, Inc., 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | CLK1, Psychiatry and Mental health, Splicing factor, Alternative splicing, Mutant, RNA splicing, Biology, Kinase activity, Temperature-sensitive mutant, Molecular biology, Nuclear localization sequence |
| الوصف: | A temperature-sensitive (ts) mutant of the CHO-K1 cell line, tsTM18, grows at 340C but not at 390C. Smu1 is the gene responsible for ts defects of tsTM18 cells. Previously, we found that the Smu1 ts defect altered the localization (as indicated by enlargement of speckles) of SRSF1 (SF2/ASF) in tsTM18 cells cultured at 390C, suggesting a functional association between Smu1 and SRSF1. Speckles are subnuclear structures that may function as storage/assembly/ modification compartments to supply splicing factors to active transcription sites. The effect of the ts defect of Smu1 on the localization of other factors related to splicing has not been characterized yet. The mechanisms underlying the enlargement of speckles of SRSF1 remain unclear. In the present study, we found that the ts defect of Smu1 affected the nuclear localization of a splicing factor, SRSF2 (SC35), and factors involved in the exon-exon junction complex, Y14 and ALY. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the ts defect of Smu1 affected alternative splicing of endogenous Clk1/ Sty and SRSF2 genes. Mammalian Clk family kinases are shown to phosphorylate serine/arginine (SR) proteins in vitro and SRSF1 in vivo. RT-PCR analysis of Clk1/Sty showed an accumulation of the truncated form lacking kinase activity in tsTM18 cells incubated at 39?C. These data indicate that an accumulation of kinase-negative Clk1/Sty may lead to alteration of the localization of factors related to splicing resulting in the enlargement of speckles. |
| تدمد: | 2161-6663 2161-6620 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99672d00589ecc1296f6ed6eaf6b4668 https://doi.org/10.4236/ajmb.2013.31005 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....99672d00589ecc1296f6ed6eaf6b4668 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21616663 21616620 |
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