Degenerate primer MOB typing of multiresistant clinical isolates of E. coli uncovers new plasmid backbones
| العنوان: | Degenerate primer MOB typing of multiresistant clinical isolates of E. coli uncovers new plasmid backbones |
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| المؤلفون: | Luis Martínez-Martínez, Belén Ruiz del Castillo, M. Pilar Garcillán-Barcia, Andrés Alvarado, Fernando de la Cruz |
| المساهمون: | Instituto de Salud Carlos III, European Commission, Red Española de Investigación en Patología Infecciosa, Gobierno de Cantabria, Fundación Leonardo Torres Quevedo, European Science Foundation, Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España), Instituto de Investigación Marqués de Valdecilla, Universidad de Cantabria |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Elsevier, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Molecular Sequence Data, Integrative and conjugative elements, Biology, Relaxase, Polymerase Chain Reaction, Plasmid, beta-Lactamases, Antibiotic resistance dissemination, Degenerate primer, Drug Resistance, Multiple, Bacterial, Escherichia coli, Humans, Replicon, Typing, Amino Acid Sequence, Molecular Biology, Phylogeny, DNA Primers, Genetics, PCR-based replicon typing, Phylogenetic tree, Base Sequence, Bacterial conjugation, Genetic Variation, Degenerate Primer MOB typing, Bacterial Typing Techniques, Mobilome, Plasmids |
| الوصف: | Degenerate Primer MOB Typing is a PCR-based protocol for the classification of γ-proteobacterial transmissible plasmids in five phylogenetic relaxase MOB families. It was applied to a multiresistant E. coli collection, previously characterized by PCR-based replicon-typing, in order to compare both methods. Plasmids from 32 clinical isolates of multiresistant E. coli (19 extended spectrum beta-lactamase producers and 13 non producers) and their transconjugants were analyzed. A total of 95 relaxases were detected, at least one per isolate, underscoring the high potential of these strains for antibiotic-resistance transmission. MOBP12 and MOBF12 plasmids were the most abundant. Most MOB subfamilies detected were present in both subsets of the collection, indicating a shared mobilome among multiresistant E. coli. The plasmid profile obtained by both methods was compared, which provided useful data upon which decisions related to the implementation of detection methods in the clinic could be based. The phylogenetic depth at which replicon and MOB-typing classify plasmids is different. While replicon-typing aims at plasmid replication regions with non-degenerate primers, MOB-typing classifies plasmids into relaxase subfamilies using degenerate primers. As a result, MOB-typing provides a deeper phylogenetic depth than replicon-typing and new plasmid groups are uncovered. Significantly, MOB typing identified 17 plasmids and an integrative and conjugative element, which were not detected by replicon-typing. Four of these backbones were different from previously reported elements. This work was supported by Ministerio de Economía y Competitividad, Instituto de Salud Carlos III – co-financed by European Development Regional Fund “A way to achieve Europe” ERDF, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015/0019), FIS (grant PI11/01117), MINECO (BFU2011-26608), and the Fundación Leonardo Torres Quevedo – University of Cantabria (grant FMV-UC 2008-01). BRC was supported by IFIMAV Cantabria, Spain. AA was partially funded by “I Plan Regional de I + D + i de Cantabria”. MPGB was funded by a JAE-Doc_2009 postdoctoral contract from Consejo Superior de Investigaciones Científicas (CSIC), which was cofinanced by the European Science Foundation. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99f2cd1ce59bd62bf2ce109ac644d275 http://hdl.handle.net/10261/130569 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....99f2cd1ce59bd62bf2ce109ac644d275 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |