Molecular study of intraspecific differences among Sauropus androgynus (L.) Merr. from Indonesia revealed by ITS region variability
| العنوان: | Molecular study of intraspecific differences among Sauropus androgynus (L.) Merr. from Indonesia revealed by ITS region variability |
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| المؤلفون: | Ike Dhiah Rochmawati, Oeke Yunita, Njoto Benarkah, Nur Aini Fadhilah |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 30, Iss 6, Pp 1212-1216 (2016) |
| بيانات النشر: | Taylor & Francis Group, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Multiple sequence alignment, Sauropus androgynus, Phylogenetic tree, Sequence analysis, lcsh:Biotechnology, Sequence alignment, sequencing, Ribosomal RNA, Biology, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, DNA sequencing, 03 medical and health sciences, 030104 developmental biology, Internal transcribed spacer, molecular study, Sauropus, lcsh:TP248.13-248.65, Botany, Biotechnology |
| الوصف: | DNA sequence analysis of the nuclear ribosomal internal transcribed spacer (ITS) region was exploited for its applications in the authentication of Sauropus androgynus (L.) Merr. as raw-material herbal medicine and supplement. Leaf samples from 12 different geographic locations in Indonesia were collected. Twelve DNA sequences from the samples were analysed. The sequences were trimmed before sequence alignment. The sequences ranged from 626 to 664 bp in length after trimming. Based on the multiple sequence alignment, a phylogenetic tree was generated using the MrBayes program. It consisted of two groups: one group comprised sequences from Trawas and the other one comprised sequences from Batu, Trawas and Surabaya. These results strongly suggest that ITS sequence analysis is a valuable tool for distinguishing intraspecific differences among S. androgynus cultivars from Indonesia. |
| اللغة: | English |
| تدمد: | 1314-3530 1310-2818 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b566f529ad14f77a572b2252a3befc5 https://doaj.org/article/dfc14bf4fc5747a981b1c31d499ee79e |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9b566f529ad14f77a572b2252a3befc5 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 13143530 13102818 |
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