Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study
| العنوان: | Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study |
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| المؤلفون: | Marco Viganò, Davide Blonna, Giuseppe M. Peretti, Antonio Marmotti, Giovanni Battista Ferrero, Filippo Castoldi, Stefania Setti, Silvia Mattia, Laura Mangiavini, Enrico Bellato, Laura de Girolamo, Davide Edoardo Bonasia |
| المصدر: | Stem Cells International, Vol 2018 (2018) Stem Cells International |
| بيانات النشر: | Hindawi Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Collagen type, lcsh:Internal medicine, Article Subject, business.industry, Mesenchymal stem cell, Scleraxis, Cell Biology, Tendon tissue, Umbilical cord, Tendon, Prolonged exposure, Andrology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, 030220 oncology & carcinogenesis, medicine, In vitro study, Corrigendum, lcsh:RC31-1245, business, Molecular Biology, Research Article |
| الوصف: | Tendon repair is a challenging procedure in orthopaedics. The use of mesenchymal stem cells (MSCs) and pulsed electromagnetic fields (PEMF) in tendon regeneration is still investigational. In this perspective, MSCs isolated from the human umbilical cord (UC) may represent a possible candidate for tendon tissue engineering. The aim of the study is to evaluate the effect of low-frequency PEMF on tenogenic differentiation of MSCs isolated from the human umbilical cord (UC-MSCs) in vitro. 15 fresh UC samples from women with healthy pregnancies were retrieved at the end of caesarean deliveries. UC samples were manually minced into small fragments (less than 4 mm length) and cultured in MSC expansion medium. Part of the UC-MSCs was subsequently cultured with PEMF and tenogenic growth factors. UC-MSCs were subjected to pulsed electromagnetic fields for 2 h/day, 4 h/day, or 8 h/day. UC-MSCs cultured with FGF-2 and stimulated with PEMF showed a greater production of collagen type I and scleraxis. The prolonged exposure to PEMF was also related to the greatest expression of tenogenic markers. Thus, the exposure to PEMF provides a positive preconditioning biophysical stimulus, which may enhance UC-MSC tenogenic potential. |
| وصف الملف: | text/xhtml |
| تدمد: | 1687-9678 1687-966X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b6790ca7947ca04178ab223c879363b https://doi.org/10.1155/2018/9048237 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9b6790ca7947ca04178ab223c879363b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16879678 1687966X |
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