SPRR3 Contributes to Aggressiveness of Pancreatic Cancer Cells via NF-κB Signaling Pathway
| العنوان: | SPRR3 Contributes to Aggressiveness of Pancreatic Cancer Cells via NF-κB Signaling Pathway |
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| المؤلفون: | Yuankang Xie, Heping Li, Baiyin Zhong, Xiansen Zhu, Rong Ye, Binhui Xie, Jianhong Zhang |
| المصدر: | BioMed Research International. 2023:1-9 |
| بيانات النشر: | Hindawi Limited, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Article Subject, General Immunology and Microbiology, General Medicine, General Biochemistry, Genetics and Molecular Biology |
| الوصف: | Pancreatic cancer remains a deadly solid tumor with worst survival, and a better understanding of the mechanisms of carcinogenesis of pancreatic cancer is critical to promote the survival of patients with pancreatic cancer. qPCR and western blot assay were used to determine the expression of SPRR3 in pancreatic cancer. Anchorage-independent growth ability, BrdU labeling, Transwell assay, and in vivo experiment were used to examine the functions of SPRR3 in aggressiveness of pancreatic cancer. Luciferase reporter assay, nucleoplasmic-separation technique, qPCR, and western blot assay were used to investigate the mechanism of SPRR3 regulating aggressiveness of pancreatic cancer. Our results showed that SPRR3 was significantly increased in pancreatic cancer, which resulted in poor survival for patients with pancreatic cancer. Further analysis showed that overexpression of SPRR3 contributed to anchorage-independent growth ability, growth rate, and invasion ability of pancreatic cancer cells. While, knockdown of SPRR3 showed the reverse results. Mechanistically, overexpression of SPRR3 can promote the transcription of NF-κB pathway, nuclear accumulation of p65, and mRNA levels of NF-κB pathway downstream genes. But, knockdown of SPRR3 induced the reverse results. The above findings clarified the important roles of SPRR3 in the progression of pancreatic cancer through NF-κB pathway. And targeting SPRR3 might be an effective strategy to therapy pancreatic cancer. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b77ba51165f227073220fdcb778b502 https://doi.org/10.1155/2023/7518744 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9b77ba51165f227073220fdcb778b502 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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