DIDS Reduces Ischemia/Reperfusion-Induced Myocardial Injury in Rats
| العنوان: | DIDS Reduces Ischemia/Reperfusion-Induced Myocardial Injury in Rats |
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| المؤلفون: | Jun Ren, Ming-Ge Ding, Xiaoming Wang, Ya-Nan Cao, Xiaole He, Yuesheng Xia, Wei-Wei Zhang, Yan Liu, Mingzhi Shen, Xihui Xu, Lin Wang, Bo Wang |
| المصدر: | Cellular Physiology and Biochemistry, Vol 35, Iss 2, Pp 676-688 (2015) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Cardiac function curve, medicine.medical_specialty, Physiology, Ischemia, Myocardial Reperfusion Injury, Apoptosis, 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, lcsh:Physiology, Rats, Sprague-Dawley, lcsh:Biochemistry, chemistry.chemical_compound, Chloride Channels, Internal medicine, Animals, Medicine, Arterial Pressure, Channel blocker, lcsh:QD415-436, Myocardial infarction, Protein kinase B, TUNEL assay, lcsh:QP1-981, business.industry, Cardiac function, medicine.disease, Ischemia/Reperfusion injury, Rats, DIDS, Endocrinology, Gene Expression Regulation, chemistry, Anesthesia, Ventricular pressure, Reactive Oxygen Species, business |
| الوصف: | Background/Aims: Anion channels such as chloride channel are known to participate in the regulation of a wide variety of cellular processes including development, differentiation, proliferation, apoptosis and regeneration. This study was designed to examine the effect of the non-selective anion channel blocker 4,4'-Diisothiocyanostilbene-2, 2'-disulfonic acid (DIDS) on cardiac function and apoptosis using a rat model of ischemia/reperfusion (I/R). Methods: Fifty male SD rats were randomly divided into the following groups including sham, I/R and I/R+DIDS (7, 14 or 28 mg/kg). In DIDS group, rats received DIDS treatment (4 ml/kg/hr) at the beginning of reperfusion for 2 hrs using a programmed micro-pump. Cardiac function was evaluated including left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) as well as positive and negative maximal derivatives of left ventricular pressure (± dP/dtmax). Myocardial infarct size was detected using the double staining with 2, 3, 5-triphenyl-2H-tetra-zolium chloride (TTC) and Evan's blue dye. DNA ladder, TUNEL assay, Bax and Bcl-2 protein levels were evaluated. Levels of ROS and Akt phosphorylation were detected. Results: I/R injury compromised cardiac function as manifested by reduced LVSP and ± dP/dtmax as well as pronounced apoptosis. I/R-induced cardiac anomalies were markedly ameliorated by DIDS. DIDS retarded I/R-induced myocardial infarct and apoptosis. In addition, DIDS ameliorated I/R-induced ROS production and Akt dephosphorylation in the heart. Conclusion: Taken together, our data revealed that DIDS may protect cardiomyocytes against I/R injury as evidenced by improved cardiac function, Bcl-2, Akt phosphorylation, and reduced myocardial apoptosis, Bax expression, ROS production and myocardial infarct size. |
| اللغة: | English |
| تدمد: | 1421-9778 1015-8987 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b949d5801ed606a7980faae8b1a6a25 http://www.karger.com/Article/FullText/369728 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9b949d5801ed606a7980faae8b1a6a25 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
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