miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/β-Catenin Signaling
| العنوان: | miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/β-Catenin Signaling |
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| المؤلفون: | Min Wang, Qin Zhou, Renqiang Yu, Yingzi Mei, Guang-Dong Fang, Ai-Ling Chen, Ying-Hui Zhang, Bing Zhang, Bei-Bei Jia, Dan-Ni Ye, Shanyu Jiang |
| المصدر: | BioMed Research International, Vol 2021 (2021) |
| بيانات النشر: | Hindawi Limited, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | A549 cell, General Immunology and Microbiology, medicine.diagnostic_test, Cell growth, Chemistry, General Medicine, Transfection, General Biochemistry, Genetics and Molecular Biology, Cell biology, Flow cytometry, Blot, Downregulation and upregulation, Apoptosis, medicine, Medicine, Secretion |
| الوصف: | Neonatal respiratory distress syndrome (NRDS) is a common disease that occurs in premature infants. However, the mechanisms underlying the disease remain unclear. microRNAs (miRNAs) have been indicated to play a crucial role in the development of NRDS. In this study, we aimed to explore the regulatory mechanisms of miR-296-5p in NRDS. The expression levels of miR-296-5p in preterm infants with NRDS were determined using quantitative reverse-transcription polymerase chain reaction (RT-qPCR). A549 cells were transfected with lentiviral vectors encoding miR-296-5p, and the transfection efficiency was determined using RT-qPCR. Flow cytometry and CCK8 assay were performed to measure apoptosis and proliferation of A549 cells, respectively. The protein levels of pulmonary surfactant SP-A (SFTPA1), SP-B, Wnt7b, and β-catenin were measured using western blotting. We demonstrated an upregulation of miR-296-5p in NRDS. The miR-296-5p was successfully overexpressed in A549 cells via lentivirus transfection, and the upregulation of miR-296-5p inhibited cell proliferation and secretion of SP-A and SP-B and also induced downregulation of the Wnt7b/β-catenin in vitro. Therefore, miR-296-5p inhibits cell proliferation and secretion of pulmonary surfactants in A549 cells via downregulation of Wnt7b/β-catenin signaling. |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c0efd19336173eab9941325c08ec64c https://doaj.org/article/a2bab4047df74670bb0da57163e74c3f |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9c0efd19336173eab9941325c08ec64c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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