Identification of Mucorales in patients with proven invasive mucormycosis by polymerase chain reaction in tissue samples
| العنوان: | Identification of Mucorales in patients with proven invasive mucormycosis by polymerase chain reaction in tissue samples |
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| المؤلفون: | Nahid Gholinejad-Ghadi, Hossein Karami, Rostam Pormosa, Seyed Reza Aghili, Mehdi Nikkhah, Tahereh Shokohi, Zahra Seifi, Iman Haghani, Laleh Vahedi Larjani, Maryam Ghasemi, Emmanuel Roilides |
| المصدر: | Mycoses. 61:909-915 |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Mucorales, medicine.medical_specialty, 030106 microbiology, Dermatology, Iran, DNA, Ribosomal, Polymerase Chain Reaction, Gastroenterology, law.invention, Young Adult, 03 medical and health sciences, Risk Factors, law, Internal medicine, DNA, Ribosomal Spacer, RNA, Ribosomal, 18S, Humans, Mucormycosis, Medicine, In patient, 18s rdna, Rhizopus arrhizus, DNA, Fungal, Polymerase chain reaction, Aged, biology, business.industry, Its region, General Medicine, Middle Aged, medicine.disease, biology.organism_classification, Infectious Diseases, Molecular Diagnostic Techniques, Child, Preschool, Rhizopus stolonifer, Female, business, Invasive Fungal Infections, Rhizopus |
| الوصف: | Background Accurate diagnosis of mucormycosis, a life-threatening fungal infection, remains a challenge for physicians. Objectives To identify the causative Mucorales in fresh clinical samples and formalin-fixed paraffin-embedded (FFPE) samples of patients with proven mucormycosis by molecular method. Patients/methods Fresh clinical samples of patients with proven mucormycosis according to the EORTC/MSG criteria admitted between 2015 and 2017 and histopathologically proven FFPE archives collected during 2004-2007 and 2015-2017 from Mazandaran University-affiliated hospitals of northern Iran were included. Seminested PCR targeting the 18S rDNA of Mucorales and ITS region was performed, and PCR products were then sequenced. Results While culture was positive only in 5 of 9 (56%) of fresh specimen cases, PCR was positive in all 9 (100%) histologically proven mucormycosis. Ten of 18 (56%) FFPE samples were PCR-positive. Overall, Mucorales PCR was positive in 19 of 27 (70%) samples. Mucorales species were Rhizopus arrhizus in 16 (84%) cases, R. arrhizus/Amylomyces rouxii in 2 (10.5%) cases and Rhizopus stolonifer in one case (5.5%). Among 27 mucormycosis cases, 25 (93%) cases were rhinocerebral, and 2 (7%) cases were disseminated. Diabetes mellitus (74%) and neutropaenia (63%) were the main risk factors. Conclusions Seminested PCR targeting 18S rDNA region of Mucorales is useful for identification of the causative agents of mucormycosis. |
| تدمد: | 1439-0507 0933-7407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c5ffe5742738deb67519a38b9819048 https://doi.org/10.1111/myc.12837 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....9c5ffe5742738deb67519a38b9819048 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390507 09337407 |
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