Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
| العنوان: | Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing |
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| المؤلفون: | Michael H. Reiskind, Loganathan Ponnusamy, Nicholas V. Travanty, Jean M Deguenon, R. Michael Roe, Ann L. Carr, Steven S. Denning, D. W. Watson, Jiwei Zhu |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Farms, Medical entomology, Science, 030106 microbiology, Zoology, medicine.disease_cause, Microbiology, Article, 03 medical and health sciences, RNA, Ribosomal, 16S, Acinetobacter radioresistens, medicine, Animals, Humans, Metabolomics, Carrion, Microbiome, Calliphoridae, Phylogeny, Multidisciplinary, biology, Ecology, Diptera, Microbiota, fungi, High-Throughput Nucleotide Sequencing, Phormia regina, Biodiversity, biology.organism_classification, 16S ribosomal RNA, 030104 developmental biology, Metabolome, Medicine, Myroides odoratimimus |
| الوصف: | The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher (P < 0.05) on external body surfaces. At the genus level, Dysgonomonas, Ignatzschineria, Acinetobacter, Vagococcus, Myroides, and Wohlfahrtiimonas were predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants. Myroides odoratimimus and Acinetobacter radioresistens are well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cb7b9626363062a1c7284a6591de59f https://doaj.org/article/ed6b5f44df394811b67999fe3a9830b8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9cb7b9626363062a1c7284a6591de59f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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