Leptin and fractalkine: novel subcutaneous cytokines in burn injury
| العنوان: | Leptin and fractalkine: novel subcutaneous cytokines in burn injury |
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| المؤلفون: | Sini Junttila, Attila Gyenesei, Helen Laycock, Elizabeth J. Want, Julia Borges Paes Lemes, Jose V. Torres-Perez, Dominic Friston, István Nagy |
| المساهمون: | British Journal of Anaesthesia, NC3Rs (National Centre for the Replacement, Refinement and Reduction of Animals in Research), Wellcome Trust, Chelsea & Westminster Health Charity |
| المصدر: | Disease Models & Mechanisms article-version (VoR) Version of Record Disease Models & Mechanisms, Vol 13, Iss 4 (2020) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Leptin, Male, Burn injury, medicine.medical_treatment, Microdialysis, Medicine (miscellaneous), lcsh:Medicine, Rats, Sprague-Dawley, Subcutaneous injection, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), Medicine, Protein Interaction Maps, 11 Medical and Health Sciences, Skin, RNAseq, 3. Good health, STAT Transcription Factors, Cytokine, Cytokines, medicine.symptom, Burns, lcsh:RB1-214, Signal Transduction, Research Article, Neuroscience (miscellaneous), Inflammation, Burn, General Biochemistry, Genetics and Molecular Biology, Fractalkine, 03 medical and health sciences, Luminex, lcsh:Pathology, Animals, Pathological, Janus Kinases, Messenger RNA, business.industry, Chemokine CX3CL1, lcsh:R, 06 Biological Sciences, 030104 developmental biology, Gene Expression Regulation, Immunology, business, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | Burn injury is a pathology underpinned by progressive and aberrant inflammation. It is a major clinical challenge to survival and quality of life. Although the complex local and disseminating pathological processes of a burn injury ultimately stem from local tissue damage, to date relatively few studies have attempted to characterise the local inflammatory mediator profile. Here, cytokine content and associated transcriptional changes were measured in rat skin for three hours immediately following induction of a scald-type (60°C, 2 min) burn injury model. Leptin (P=0.0002) and fractalkine (P=0.0478) concentrations were significantly elevated post-burn above pre-burn and control site values, coinciding with the development of burn site oedema and differential expression of leptin mRNA (P=0.0004). Further, gene sequencing enrichment analysis indicated cytokine-cytokine receptor interaction (P=1.45×10−6). Subsequent behavioural studies demonstrated that, following subcutaneous injection into the dorsum of the paw, both leptin and fractalkine induced mechanical allodynia, heat hyperalgesia and the recruitment of macrophages. This is the first report of leptin elevation specifically at the burn site, and the first report of fractalkine elevation in any tissue post-burn which, together with the functional findings, calls for exploration of the influence of these cytokines on pain, inflammation and burn wound progression. In addition, targeting these signalling molecules represents a therapeutic potential as early formative mediators of these pathological processes. Summary: Burn injury is associated with increased levels of leptin and fractalkine in the skin. Signalling by these cytokines leads to macrophage accumulation and hypersensitivity to heat and mechanical stimuli. |
| تدمد: | 1754-8411 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cffc5f0dfb23f00ec526645d783bb9c https://pubmed.ncbi.nlm.nih.gov/32127397 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9cffc5f0dfb23f00ec526645d783bb9c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17548411 |
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