miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1
| العنوان: | miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1 |
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| المؤلفون: | Jun Luo, Huiling Qiu, Liuan Ma, Juan J. Loor, Zhi Chen, Kang Kang, Deming Gou, Shuang Sun |
| المصدر: | International Journal of Molecular Sciences; Volume 17; Issue 11; Pages: 1909 International Journal of Molecular Sciences, Vol 17, Iss 11, p 1909 (2016) International Journal of Molecular Sciences |
| بيانات النشر: | MDPI AG, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | LRP6, 0301 basic medicine, miR-30e-5p, miR-15a, fat metabolism, YAP1, Biology, Article, Catalysis, lcsh:Chemistry, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Mammary Glands, Animal, Western blot, Adipocyte, microRNA, medicine, Animals, Humans, Physical and Theoretical Chemistry, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, Adaptor Proteins, Signal Transducing, Regulation of gene expression, Gene knockdown, Fatty acid metabolism, medicine.diagnostic_test, Goats, Fatty Acids, Organic Chemistry, Epithelial Cells, Lipid metabolism, General Medicine, Molecular biology, Up-Regulation, Computer Science Applications, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, lcsh:Biology (General), lcsh:QD1-999, chemistry, Low Density Lipoprotein Receptor-Related Protein-6, Female |
| الوصف: | MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating (“dry”) periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (LRP6) and Yes associated protein 1 (YAP1) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC. Moreover, we demonstrated that the overexpression of miR-30e-5p and miR-15a in GMEC promoted fat metabolism while their knockdown impaired fat metabolism. These findings extend the discovery of a key role of miR-30e-5p and miR-15a in mediating adipocyte differentiation by suggesting a role in promoting milk fat synthesis. In conclusion, our findings indicate that miR-30e-5p, together with miR-15a, represses expression of LRP6 and promotes fat metabolism in GMEC. The data expanded our knowledge on the function of miRNAs in milk fat metabolism and synthesis in ruminant mammary cells. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d624db55964e08f48d3ff2f6d58453a https://doi.org/10.3390/ijms17111909 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9d624db55964e08f48d3ff2f6d58453a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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